Identification of mature peptides from pban and capa genes of the moths Heliothis peltigera and Spodoptera littoralis
| Autor: | Reinhard Predel, Yuval Salts, Miriam Altstein, Michael Davidovitch, Susanne Neupert, Lapo Ragionieri, Burak Özbagci |
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| Rok vydání: | 2017 |
| Předmět: |
Central Nervous System
Male 0301 basic medicine Signal peptide Physiology Peltigera Moths Spodoptera Biochemistry Transcriptome 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Endocrinology Gene expression Botany Animals Spodoptera littoralis Gene chemistry.chemical_classification Spatial Analysis biology Neuropeptides biology.organism_classification Amino acid 030104 developmental biology chemistry Insect Proteins Female Heliothis peltigera 030217 neurology & neurosurgery |
| Zdroj: | Peptides |
| ISSN: | 0196-9781 |
| Popis: | By transcriptome analysis, we identified PBAN and CAPA precursors in the moths Spodoptera littoralis and Heliothis peltigera which are among the most damaging pests of agriculture in tropical and subtropical Africa as well as in Mediterranean countries. A combination of mass spectrometry and immunocytochemistry was used to identify mature peptides processed from these precursors and to reveal their spatial distribution in the CNS. We found that the sites of expression of pban genes, the structure of PBAN precursors and the processed neuropeptides are very similar in noctuid moths. The sequence of the diapause hormone (DH; tryptopyrokinin following the signal peptide), however, contains two N-terminal amino acids more than expected from comparison with already published sequences of related species. Capa genes of S. littoralis and H. peltigera encode, in addition to periviscerokinins, a tryptopyrokinin showing sequence similarity with DH, which is the tryptopyrokinin of the pban gene. CAPA peptides, which were not known from any noctuid moth so far, are produced in cells of abdominal ganglia. The shape of the release sites of these hormones in H. peltigera represents an exceptionally derived trait state and does not resemble the well-structured abdominal perisympathetic organs which are known from many other insects. Instead, axons of CAPA cells extensively ramify within the ventral diaphragm. The novel information regarding the sequences of all mature peptides derived from pban and capa genes of H. peltigera and S. littoralis now enables a detailed analysis of the bioactivity and species-specificity of the native peptides, especially those from the hitherto unknown capa genes, and to explore their interactions with PBAN/DH receptors. |
| Databáze: | OpenAIRE |
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