l -Thyroxine promotes a proliferative airway smooth muscle phenotype in the presence of TGF-β1
| Autor: | I. Sophie T. Bos, Mark H. Menzen, Saeideh Naeimi, Bart G. J. Dekkers, Herman Meurs, Andrew J. Halayko, Goudarz Sadeghi Hashjin |
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| Přispěvatelé: | Molecular Pharmacology, Groningen Research Institute for Asthma and COPD (GRIAC) |
| Rok vydání: | 2015 |
| Předmět: |
Physiology
airway smooth muscle proliferation Gene Expression Muscle Proteins THYROID-HORMONE transforming growth factor-beta 1 Extracellular matrix Receptor Triiodothyronine Phenotype Airway Remodeling medicine.symptom Pulmonary and Respiratory Medicine medicine.medical_specialty MAP Kinase Signaling System Myocytes Smooth Muscle FIBRONECTIN Down-Regulation Bronchi Inflammation Biology OBSTRUCTIVE PULMONARY-DISEASE Cell Line Transforming Growth Factor beta1 INFLAMMATION HYPERRESPONSIVENESS Physiology (medical) Internal medicine EXTRACELLULAR-MATRIX medicine Humans MEDIATE ENHANCEMENT Cell Proliferation Asthma airway smooth muscle contractile protein expression RECEPTOR Cell Biology Integrin alphaVbeta3 medicine.disease L-thyroxine Fibronectin TRIIODOTHYRONINE-INDUCED THYROTOXICOSIS Thyroxine Endocrinology Immunology biology.protein ASTHMA Transforming growth factor |
| Zdroj: | American Journal of Physiology-Lung Cellular and Molecular Physiology, 308(3), L301-L306. AMER PHYSIOLOGICAL SOC |
| ISSN: | 1522-1504 1040-0605 |
| Popis: | Hypothyroidism may reduce, whereas hyperthyroidism may aggravate, asthma symptoms. The mechanisms underlying this relationship are largely unknown. Since thyroid hormones have central roles in cell growth and differentiation, we hypothesized that airway remodeling, in particular increased airway smooth muscle (ASM) mass, may be involved. To address this hypothesis, we investigated the effects of triiodothyronine (T3) and l-thyroxine (T4) in the absence and presence of the profibrotic transforming growth factor (TGF)-β1 on human ASM cell phenotype switching. T3 (1–100 nM) and T4 (1–100 nM) did not affect basal ASM proliferation. However, when combined with TGF-β1 (2 ng/ml), T4 synergistically increased the proliferative response, whereas only a minor effect was observed for T3. In line with a switch from a contractile to a proliferative ASM phenotype, T4 reduced the TGF-β1-induced contractile protein expression by ∼50%. Cotreatment with T3 reduced TGF-β1-induced contractile protein expression by ∼25%. The synergistic increase in proliferation was almost fully inhibited by the integrin αvβ3 antagonist tetrac (100 nM), whereas no significant effects of the thyroid receptor antagonist 1–850 (3 μM) were observed. Inhibition of MEK1/2, downstream of the integrin αvβ3, also inhibited the T4- and TGF-β1-induced proliferative responses. Collectively, the results indicate that T4, and to a lesser extent T3, promotes a proliferative ASM phenotype in the presence of TGF-β1, which is predominantly mediated by the membrane-bound T4 receptor αvβ3. These results indicate that thyroid hormones may enhance ASM remodeling in asthma, which could be of relevance for hyperthyroid patients with this disease. |
| Databáze: | OpenAIRE |
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