DNA extraction from filter-paper spots of vaginal samples collected after sexual violence

Autor: Fernanda Aparecida Costa, Denise Rocha Pitta, Elisabete Aparecida Campos, Arlete Fernandes, Daniela Angerame Yela, Vanessa M. Campos
Rok vydání: 2013
Předmět:
Zdroj: International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics. 126(1)
ISSN: 1879-3479
Popis: Objective To detect the presence of male DNA in vaginal samples collected from survivors of sexual violence and stored on filter paper. Methods A pilot study was conducted to evaluate 10 vaginal samples spotted on sterile filter paper: 6 collected at random in April 2009 and 4 in October 2010. Time between sexual assault and sample collection was 4–48 hours. After drying at room temperature, the samples were placed in a sterile envelope and stored for 2–3 years until processing. DNA extraction was confirmed by polymerase chain reaction for human β-globin, and the presence of prostate-specific antigen (PSA) was quantified. The presence of the Y chromosome was detected using primers for sequences in the TSPY (Y7/Y8 and DYS14) and SRY genes. Results β-Globin was detected in all 10 samples, while 2 samples were positive for PSA. Half of the samples amplified the Y7/Y8 and DYS14 sequences of the TSPY gene and 30% amplified the SRY gene sequence of the Y chromosome. Four male samples and 1 female sample served as controls. Conclusion Filter-paper spots stored for periods of up to 3 years proved adequate for preserving genetic material from vaginal samples collected following sexual violence.
Databáze: OpenAIRE
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