The fatty acid synthase fasn-1 acts upstream of WNK and Ste20/GCK-VI kinases to modulate antimicrobial peptide expression in C. elegans epidermis
| Autor: | Marina Kniazeva, Nathalie Pujol, Kwang-Zin Lee, Min Han, Jonathan J. Ewbank |
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| Přispěvatelé: | Centre d'Immunologie de Marseille - Luminy (CIML), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Howard Hughes Medical Institute [Chevy Chase] (HHMI), Howard Hughes Medical Institute (HHMI), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
MESH: Signal Transduction
Antifungal Agents p38 Mitogen-Activated Protein Kinases chemistry.chemical_compound MESH: Fatty Acid Synthetase Complex 0302 clinical medicine WNK Lysine-Deficient Protein Kinase 1 Gene expression Candida albicans Homeostasis MESH: Animals Regulator gene 0303 health sciences Kinase MESH: Antimicrobial Cationic Peptides MESH: Caenorhabditis elegans Proteins Water-Electrolyte Balance MESH: Gene Expression Regulation 3. Good health Fatty acid synthase Infectious Diseases Biochemistry MESH: Homeostasis Hypocreales [SDV.IMM]Life Sciences [q-bio]/Immunology MESH: Immunity Innate Research Paper Signal Transduction Microbiology (medical) p38 mitogen-activated protein kinases Immunology Biology Protein Serine-Threonine Kinases Microbiology MESH: Protein-Serine-Threonine Kinases 03 medical and health sciences MESH: Caenorhabditis elegans Humans Animals Caenorhabditis elegans Caenorhabditis elegans Proteins Fatty acid synthesis 030304 developmental biology Innate immune system Fatty acid metabolism Immunity Innate MESH: p38 Mitogen-Activated Protein Kinases chemistry Gene Expression Regulation biology.protein MESH: Water-Electrolyte Balance Parasitology Epidermis Fatty Acid Synthases MESH: Epidermis 030217 neurology & neurosurgery Antimicrobial Cationic Peptides |
| Zdroj: | Blood Blood, American Society of Hematology, 2010, 1 (3), pp.113-22. ⟨10.4161/viru.1.3.10974⟩ Blood, 2010, 1 (3), pp.113-22. ⟨10.4161/viru.1.3.10974⟩ |
| ISSN: | 0006-4971 1528-0020 |
| DOI: | 10.4161/viru.1.3.10974⟩ |
| Popis: | International audience; An important part of the innate immune response of the nematode C. elegans to fungal infection is the rapid induction of antimicrobial peptide gene expression. One of these genes, nlp‑29, is expressed at a low level in adults under normal conditions. Its expression is up-regulated in the epidermis by infection with Drechmeria coniospora, but also by physical injury and by osmotic stress. For infection and wounding, the induction is dependent on a p38 MAP kinase cascade, but for osmotic stress, this pathway is not required. To characterize further the pathways that control the expression of nlp‑29, we carried out a genetic screen for negative regulatory genes. We isolated a number of Peni (peptide expression no infection) mutants and cloned one. It corresponds to fasn‑1, the nematode ortholog of vertebrate fatty acid synthase. We show here that a pathway involving fatty acid synthesis and the evolutionary conserved wnk‑1 and gck‑3/Ste20/GCK‑VI kinases modulates nlp‑29 expression in the C. elegans epidermis, independently of p38 MAPK signaling. The control of the antimicrobial peptide gene nlp‑29 thus links different physiological processes, including fatty acid metabolism, osmoregulation, maintenance of epidermal integrity and the innate immune response to infection. |
| Databáze: | OpenAIRE |
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