Fluorescence-based kinetic analysis of miniaturized protein microarrays
| Autor: | Levi A. Gheber, Heman Kumar Ramiya Ramesh Babu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Computer science Microfluidics Protein Array Analysis Biomedical Engineering Biophysics Biosensing Techniques 02 engineering and technology Signal Multiplexing Fluorescence Reduction (complexity) Mice 03 medical and health sciences Software portability Electrochemistry Miniaturization Animals Sensitivity (control systems) business.industry Equipment Design General Medicine Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology Kinetics 030104 developmental biology Immunoglobulin G Protein microarray Rabbits 0210 nano-technology business Antibodies Immobilized Chickens Computer hardware Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 122:290-299 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2018.09.051 |
| Popis: | Ideal monitoring devices should enjoy a combination of characteristics, e.g. high sensitivity, multiplexing, portability, short time-to-result (TTR). Typically, no device meets all of these demands since some of them are contradictory, to some extent. Herein, we present a miniaturized platform based on fluorescent detection, which is sensitive, readily allows multiplexing, and allows real-time monitoring of the signal, thus allowing extraction of kinetic information as well as drastic reduction of TTR. This is achieved via miniaturization of active spots, integration with microfluidics, and algorithmic approaches. We validate its performance by comparing with evanescent field excitation, which obtains similar results, however without the addition of the necessary complex hardware. |
| Databáze: | OpenAIRE |
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