The C5a receptor is expressed by human renal proximal tubular epithelial cells
| Autor: | T. R. Welch, R. Zahedi, B. H. Ault, A. Khan, M. Frenzke, Rick A. Wetsel, Michael C. Braun, Alvin E. Davis |
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| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Cell type
Glomerular Mesangial Cell Immunology Urinary Bladder Complement Enzyme-Linked Immunosorbent Assay Biology C5a receptor Kidney Tubules Proximal Antigens CD medicine Immunology and Allergy Humans RNA Messenger RNA Neoplasm Receptor Fluorescent Antibody Technique Indirect Growth Substances Kidney Tubules Distal Receptor Anaphylatoxin C5a Cells Cultured Kidney urogenital system Reverse Transcriptase Polymerase Chain Reaction Monocyte Epithelial Cells Muscle Smooth U937 Cells Flow Cytometry Epithelium Glomerular Mesangium Receptors Complement medicine.anatomical_structure Immunohistochemistry Cytokines Ureter Cell Division |
| Popis: | SUMMARYThe C5a receptor is expressed by a variety of cell types. These studies demonstrate by immunohistochemistry that the receptor is present on the surface of proximal and distal tubular epithelial cells from normal kidney. In addition, the receptor was detected on transitional epithelial cells of the ureter and bladder. Primary proximal tubular cultures and a proximal tubular cell line both also expressed the C5a receptor, as demonstrated by immunofluorescence and by FACS analysis. The presence of mRNA encoding the receptor was confirmed by reverse transcriptase-polymerase chain reaction analysis. As opposed to its effect on glomerular mesangial cells, the receptor did not mediate a proliferative response by the proximal tubular cells. C5a also did not enhance the synthesis/secretion of transforming growth factor-beta 1, monocyte chemoattractant protein-1, platelet-derived growth factor-AB or tumour necrosis factor-alpha by cultured proximal tubular cells. Therefore, although the C5a receptor clearly is expressed by proximal tubular cells, clarification of its functional relevance on this cell type awaits further studies. |
| Databáze: | OpenAIRE |
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