Two novel presenilin-1 mutations (Y256S and Q222H) are associated with early-onset Alzheimer’s disease
| Autor: | Kevin Taddei, Justin Fonte, Francoise Suard, Christopher M. Fisher, Domizio Suva, Catriona McLean, Pankaj D. Mehta, Anastazija Gnjec, Colin L. Masters, Judith Miklossy, Joseph Ghika, William S. Brooks, Ralph N. Martins, Giuseppe Verdile |
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| Rok vydání: | 2003 |
| Předmět: |
Adult
Aging Glutamine Blotting Western DNA Mutational Analysis Mutant Mutation Missense Enzyme-Linked Immunosorbent Assay Plaque Amyloid Biology medicine.disease_cause Presenilin Degenerative disease Alzheimer Disease Presenilin-1 Serine medicine Humans Missense mutation Histidine Early-onset Alzheimer's disease Gene Family Health Genetics Mutation Amyloid beta-Peptides General Neuroscience Brain Membrane Proteins medicine.disease Immunohistochemistry Peptide Fragments Pedigree Amino Acid Substitution Tyrosine Female Neurology (clinical) Geriatrics and Gerontology Alzheimer's disease Developmental Biology |
| Zdroj: | Neurobiology of Aging. 24:655-662 |
| ISSN: | 0197-4580 |
| Popis: | Mutations in the gene encoding presenilin 1 (PS-1) account for 50% of early-onset familial Alzheimer’s disease (EOFAD) cases. In this study, we identified two missense mutations in the coding sequence of the presenilin (PS-1) gene in two EOFAD pedigrees. AD was confirmed in one pedigree by autopsy. Mutation analysis of PCR products amplified from genomic DNA templates showed two novel PS-1 mutations resulting in Gln222His and Tyr256Ser. The two novel mutations are located within predicted transmembrane domains five (TM-5) and six (TM-6), respectively, and are associated with very early ages of onset. The Tyr256Ser is associated with one of the youngest age of AD onset, 25 years, which is consistent with a drastic change in function of the altered PS-1 protein. A morphometric analysis of the cortical degenerative changes of the Tyr256Ser case, showed severe involvement of the primary motor cortex, which correlated well with the pyramidal changes, including tetraspasticity. Immunoblot analysis showed the Tyr256Ser case had the greatest expression of Aβ1–40 and Aβ1–42, which was confirmed by ELISA, compared to other PS-1 mutant FAD cases and age-matched controls and, thus, contributes to the severity of the disease pathology. |
| Databáze: | OpenAIRE |
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