Cloning of a gene involved in rRNA precursor processing and 23S rRNA cleavage in Rhodobacter capsulatus
| Autor: | J Fritsch, E Kordes, Susanne Jock, Gabriele Klug, F Bosch |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Molecular Sequence Data
Mutant Biology Molecular cloning medicine.disease_cause Microbiology Rhodobacter capsulatus 23S ribosomal RNA Endoribonucleases medicine Cloning Molecular RNA Processing Post-Transcriptional Internal transcribed spacer Bacteriochlorophylls Molecular Biology Escherichia coli Rhodobacter Base Sequence Genetic Complementation Test Wild type Ribosomal RNA biology.organism_classification Molecular biology RNA Ribosomal 23S Chloramphenicol Genes Bacterial Mutation Nucleic Acid Conformation Research Article |
| Zdroj: | Journal of Bacteriology. 176:1121-1127 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.176.4.1121-1127.1994 |
| Popis: | In Rhodobacter capsulatus wild-type strains, the 23S rRNA is cleaved into [16S] and [14S] rRNA molecules. Our data show that a region predicted to form a hairpin-loop structure is removed from the 23S rRNA during this processing step. We have analyzed the processing of rRNA in the wild type and in the mutant strain Fm65, which does not cleave the 23S rRNA. In addition to the lack of 23S rRNA processing, strain Fm65 shows impeded processing of a larger 5.6-kb rRNA precursor and slow maturation of 23S and 16S rRNAs from pre-23S and pre-16S rRNA species. Similar effects have also been described previously for Escherichia coli RNase III mutants. Processing of the 5.6-kb precursor was independent of protein synthesis, while the cleavage of 23S rRNA to generate 16S and 14S rRNA required protein synthesis. We identified a DNA fragment of the wild-type R. capsulatus chromosome that conferred normal processing of 5.6-kb rRNA and 23S rRNA when it was expressed in strain Fm65. |
| Databáze: | OpenAIRE |
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