Structural Investigation of the Lipopolysaccharide from Acinetobacter Haemolyticus Strain NCTC 10305 (ATCC 17906, DNA Group 4)
| Autor: | Sven Müller-Loennies, Bent O. Petersen, Otto Holst, Sergey V. Meshkov, Evgeny Vinogradov, Jane Thomas-Oates, Helmut Brade |
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| Rok vydání: | 1997 |
| Předmět: |
Lipopolysaccharides
chemistry.chemical_classification Acinetobacter biology Strain (chemistry) Oligosaccharides Nuclear magnetic resonance spectroscopy Carbohydrate biology.organism_classification Biochemistry Acinetobacter haemolyticus Mass Spectrometry Dephosphorylation Lipid A chemistry Pyranose lipids (amino acids peptides and proteins) Hexose |
| Zdroj: | European Journal of Biochemistry. 247:82-90 |
| ISSN: | 1432-1033 0014-2956 |
| Popis: | The structure of the lipopolysaccharide (LPS) from Acinetobacter haemolyticus strain NCTC 10305 (DNA group 4) was elucidated by means of analytical chemistry, NMR spectroscopy and fast-atom-bombardment mass spectrometry. Several oligosaccharides were obtained after deacylation or successive de-O-acylation, dephosphorylation, reduction, and de-N-acylation of LPS. In the major fraction of the LPS, the core is attached to the lipid A through D-glycero-D-talo-2-octulopyranosonic acid (Ko), whereas in a minor fraction (20%) Ko is replaced by 3-deoxy-D-manno-octulopyranosonic acid (Kdo). The structures of the phosphorylated carbohydrate backbones of these LPS fractions are [structure: see text] with Dha = 3-deoxy-D-lyxo-2-heptulosaric acid, Sug = sugar and is Ko in a major fraction and Kdo in a minor fraction. All sugar residues have the D-configuration and are present in the pyranose form. Mass spectrometry of de-O-acylated LPS revealed the presence of an additional hexose residue in minor amounts, the position and nature of which could not be identified. |
| Databáze: | OpenAIRE |
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