1-phenyl 2-thiourea (PTU) activates autophagy in zebrafish embryos
| Autor: | Alvin C.H. Ma, Xiang Ke Chen, Raymond Chuen-Chung Chang, Joseph Kwan |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Autophagosome endocrine system animal structures Tyrosinase Green fluorescent protein 03 medical and health sciences chemistry.chemical_compound Sequestosome 1 Autophagy Animals Phosphatidylinositol Bovine serum albumin education Molecular Biology Zebrafish education.field_of_study 030102 biochemistry & molecular biology biology Autophagosomes Thiourea Chloroquine Cell Biology biology.organism_classification Class III Phosphatidylinositol 3-Kinases Cell biology 030104 developmental biology chemistry embryonic structures biology.protein Lysosomes Research Paper |
| Zdroj: | Autophagy |
| ISSN: | 1554-8635 1554-8627 |
| Popis: | 1-phenyl 2-thiourea (PTU) is a Tyr (tyrosinase) inhibitor that is extensively used to block pigmentation and improve optical transparency in zebrafish (Danio rerio) embryo. Here, we reported a previously undescribed effect of PTU on macroautophagy/autophagy in zebrafish embryos. Upon 0.003% PTU treatment, aberrant autophagosome and autolysosome formation, accumulation of lysosomes, and elevated autophagic flux were observed in various tissues and organs of zebrafish embryos, such as skin, brain, and muscle. Similar to PTU treatment, autophagic activation and lysosomal accumulation were also observed in the somatic tyr mutant zebrafish embryos, which suggest that Tyr inhibition may contribute to PTU-induced autophagic activation. Furthermore, we demonstrated that autophagy contributes to pigmentation inhibition, but is not essential to the PTU-induced pigmentation inhibition. With the involvement of autophagy in a wide range of physiological and pathological processes and the routine use of PTU in zebrafish research of autophagy-related processes, these observations raise a novel concern in autophagy-related studies using PTU-treated zebrafish embryos. Abbreviations: 3-MA: 3-methyladenine; Atg: autophagy-related; BSA: bovine serum albumin; CHT: caudal hematopoietic tissue; CQ: chloroquine; GFP: green fluorescent protein; hpf: hour-post-fertilization; Map1lc3/Lc3: microtubule-associated protein 1 light chain 3; NGS: normal goat serum; PtdIns3K: class III phosphatidylinositol 3-kinase; PTU: 1-phenyl 2-thiourea; RFP: red fluorescent protein; Sqstm1: sequestosome 1; tyr: tyrosinase. |
| Databáze: | OpenAIRE |
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