Profile of the SeptiCyte™ LAB gene expression assay to diagnose infection in critically ill patients
| Autor: | M. J. M. Bonten, Diana M. Verboom, Marlies E. Koster-Brouwer, Olaf L. Cremer, Meri R.J. Varkila |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
diagnosis Critical Illness Sepsis/diagnosis Review Systemic inflammation Research Support law.invention Gene Expression Profiling/instrumentation Pathology and Forensic Medicine Transcriptome Sepsis sepsis 03 medical and health sciences 0302 clinical medicine law Gene expression medicine Genetics Journal Article gene expression assay Humans Non-U.S. Gov't Molecular Biology Polymerase chain reaction Whole blood SeptiCyte™ LAB Critically ill business.industry Gene Expression Profiling Research Support Non-U.S. Gov't biomarkers medicine.disease Molecular medicine infection 030104 developmental biology 030220 oncology & carcinogenesis Immunology SeptiCyte (TM) LAB Molecular Medicine medicine.symptom business |
| Zdroj: | Expert Review of Molecular Diagnostics, 19(2), 95. Expert Reviews Ltd. |
| ISSN: | 1473-7159 |
| Popis: | Sepsis is a severe and frequently occurring clinical syndrome, caused by the inflammatory response to infections. Recent studies on the human transcriptome during sepsis have yielded several gene-expression assays that might assist physicians during clinical assessment of patients suspected of sepsis. SeptiCyte™ LAB (Immunexpress, Seattle, WA) is the first gene expression assay that was cleared by the FDA in the United States to distinguish infectious from non-infectious causes of systemic inflammation in critically ill patients. The test consists of the simultaneous amplification of four RNA transcripts (CEACAM4, LAMP1, PLAC8, and PLA2G7) in whole blood using a quantitative real time PCR reaction. This review provides an overview of the challenges in the diagnosis of sepsis, the development of gene expression signatures, and a detailed description of available clinical performance studies evaluating SeptiCyte™ LAB. |
| Databáze: | OpenAIRE |
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