ALIX and ESCRT-III Coordinately Control Cytokinetic Abscission during Germline Stem Cell Division In Vivo
| Autor: | Eikenes, Åsmund, Malerød, Lene, Christensen, Anette Lie, Steen, Chloé, Mathieu, Juliette, Nezis, Ioannis, Liestol, Knut, Huynh, Jean-René, Stenmark, Harald, Haglund, Kaisa, Copenhaver, Gregory |
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| Přispěvatelé: | University of Oslo (UiO), Collège de France (CdF), Institut Jacques Monod (IJM (UMR_7592)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Collège de France (CdF (institution)) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Cancer Research
Cell division lcsh:QH426-470 [SDV]Life Sciences [q-bio] Biology ESCRT 03 medical and health sciences 0302 clinical medicine Abscission Genetics Molecular Biology Genetics (clinical) Ecology Evolution Behavior and Systematics ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences Cell cycle Cell biology QR Midbody lcsh:Genetics Stem cell division Stem cell 030217 neurology & neurosurgery Cytokinesis Research Article |
| Zdroj: | PLoS Genetics PLoS Genetics, Public Library of Science, 2015, 11 (1), pp.e1004904. ⟨10.1371/journal.pgen.1004904⟩ PLoS Genetics, Vol 11, Iss 1, p e1004904 (2015) |
| ISSN: | 1553-7390 1553-7404 |
| DOI: | 10.1371/journal.pgen.1004904⟩ |
| Popis: | Abscission is the final step of cytokinesis that involves the cleavage of the intercellular bridge connecting the two daughter cells. Recent studies have given novel insight into the spatiotemporal regulation and molecular mechanisms controlling abscission in cultured yeast and human cells. The mechanisms of abscission in living metazoan tissues are however not well understood. Here we show that ALIX and the ESCRT-III component Shrub are required for completion of abscission during Drosophila female germline stem cell (fGSC) division. Loss of ALIX or Shrub function in fGSCs leads to delayed abscission and the consequent formation of stem cysts in which chains of daughter cells remain interconnected to the fGSC via midbody rings and fusome. We demonstrate that ALIX and Shrub interact and that they co-localize at midbody rings and midbodies during cytokinetic abscission in fGSCs. Mechanistically, we show that the direct interaction between ALIX and Shrub is required to ensure cytokinesis completion with normal kinetics in fGSCs. We conclude that ALIX and ESCRT-III coordinately control abscission in Drosophila fGSCs and that their complex formation is required for accurate abscission timing in GSCs in vivo. Author Summary Cytokinesis, the final step of cell division, concludes with a process termed abscission, during which the two daughter cells physically separate. In spite of their importance, the molecular machineries controlling abscission are poorly characterized especially in the context of living metazoan tissues. Here we provide molecular insight into the mechanism of abscission using the fruit fly Drosophila melanogaster as a model organism. We show that the scaffold protein ALIX and the ESCRT-III component Shrub are required for completion of abscission in Drosophila female germline stem cells (fGSCs). ESCRT-III has been implicated in topologically similar membrane scission events as abscission, namely intraluminal vesicle formation at endosomes and virus budding. Here we demonstrate that ALIX and Shrub co-localize and interact to promote abscission with correct timing in Drosophila fGSCs. We thus show that ALIX and ESCRT-III coordinately control abscission in Drosophila fGSCs cells and report an evolutionarily conserved function of the ALIX/ESCRT-III pathway during cytokinesis in a multi-cellular organism. |
| Databáze: | OpenAIRE |
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