Effect of the Freeze-Drying Process on the Physicochemical and Microbiological Properties of Mexican Kefir Grains

Autor: Alicia Águeda Conde-Islas, Galo R. Urrea-García, Denis Cantú-Lozano, Maribel Jiménez-Fernández, Guadalupe Luna-Solano
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Processes, Vol 7, Iss 3, p 127 (2019)
Processes
Volume 7
Issue 3
ISSN: 2227-9717
Popis: The purpose of this study was to investigate how the properties of Mexican kefir grains (MKG) are affected by the operating parameters used in the freeze-drying process. The factors investigated were the freezing time (3&ndash
9 h), freezing temperature (&minus
20 to &minus
80 °
C), pressure (0.2&ndash
0.8 mbar), and lyophilization time (5&ndash
20 h). The maximum range of change and one-way analysis of variance showed that lyophilization time and freezing time significant affects (p <
0.05) the response variables, residual moisture content and water activity, and pressure had a significant effect on the color difference and survival rate of probiotic microorganisms. The best drying conditions were a freezing time of 3 h, a freezing temperature of &minus
20 °
C, a pressure of 0.6 mbar, and a lyophilization time of 15 h. Under these conditions, we obtained a product with residual moisture content below 6%, water activity below 0.2, and survival rates above 8.5 log cfu per gram of lactic acid bacteria and above 8.6 log for yeast.
Databáze: OpenAIRE