Matrix effect marker for multianalyte analysis by LC–MS/MS in biological samples
| Autor: | Jesús Muñoz-Guerra, Gloria Muñoz, Eva Tudela |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Doping in Sports
Ions Analyte Chromatography Chemistry Clinical Biochemistry Extraction (chemistry) Ion suppression in liquid chromatography–mass spectrometry Cell Biology General Medicine Urine Mass spectrometry Biochemistry Analytical Chemistry Models Chemical Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Lc ms ms Humans Sample preparation Diuretics Glucocorticoids Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography B. 901:98-106 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2012.06.007 |
| Popis: | Matrix effects (ion suppression/enhancement) are a well-observed phenomenon in analyses of biological matrices by high-performance liquid chromatography-mass spectrometry (LC-MS). However, few simple solutions for detecting and minimizing these adverse effects have been described so far in multianalyte analysis, especially in the field of doping control. This study describes an exhaustive characterization of matrix effects in one hundred urine samples fortified with 41 analytes (glucocorticoids and diuretics). It introduces a novel marker to identify samples in which the reliability of the results is compromised because of acute ion suppression. This new strategy strengthens the rigor of the analysis for screening purposes. Once the matrix effect is identified, a selective sample preparation is introduced to minimize the adverse ion suppression effect. That selective extraction together with the use of a deuterated internal standard permits enhancing the ruggedness of the estimation of glucocorticoid concentration in urine. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect