Exploring potential inhibitor of SARS-CoV2 replicase from FDA approved drugs using insilico drug discovery methods
| Autor: | Anshuman Chandra, Ali S. Alqahtani, Vaishali Gurjar, Mohammad Z. Ahmed, Imteyaz Qamar, Nagendra Singh |
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| Rok vydání: | 2021 |
| Předmět: |
Drug
In silico media_common.quotation_subject 030303 biophysics RNA-dependent RNA polymerase Pharmacology Molecular Dynamics Simulation Antiviral Agents Virus 03 medical and health sciences Structural Biology In vivo Drug Discovery Medicine Humans Protease Inhibitors Molecular Biology media_common 0303 health sciences Virtual screening business.industry Drug discovery SARS-CoV-2 General Medicine COVID-19 Drug Treatment Molecular Docking Simulation Drug repositioning RNA Viral business |
| Zdroj: | Journal of Biomolecular Structure and Dynamics |
| ISSN: | 1538-0254 |
| Popis: | Severe Acute Respiratory Syndrome Corona Virus-2 (SARS-CoV2) is responsible for fetal pneumonia called COVID19. SARS-CoV2 emerged in Wuhan, Hubei Province of China in December 2019. The COVID19 pandemic has now gripped the entire world with more than 70 million cases and over 1.5 million deaths so far. There no treatment option for COVID19 is in term of a drug or vaccine is currently available. Therefore drug repurposing may only provide a quick method for utilizing existing drugs for a therapeutic option. The virus genome contains several non-structural proteins (NSP) which serve as target for designing of antiviral agents. NSP9 of SARS-CoV2 encodes for a replicase enzyme which is essential for the virus replication in the host cell. In search of potent inhibitors, we have screened FDA approved drugs against NSP9 using in silico methods. Five drugs fluspirilene, troglitazone, alvesco, dihydroergotoxine and avodart were found to have highest affinities with the replicase. The molecular dynamics simulation (MDS) studies demonstrated strong drugs binding and stable NSP9-drugs complexes formation. The findings are also strongly supported by root-mean-square deviation, root-mean-square fluctuation, radius of gyration, and hydrogen bond analysis of the complexes. Principal component analysis showed the stable conformation of NSP9 upon drug binding. It could be inferred that these five drugs individually or in combinations may be used as potential inhibitors of NSP9 of SARS-CoV-2 after exploring their in vivo antiviral potential.Communicated by Ramaswamy H. Sarma. |
| Databáze: | OpenAIRE |
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