C1q/TNF-related protein 9 improves the anti-contractile effects of perivascular adipose tissue via the AMPK-eNOS pathway in diet-induced obese mice
| Autor: | Xiaodong Sun, Qingjie Mu, Mingxia Shao, Fang Han, Xiaotong Jiao, Yang Zhang, Ningning Hou |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Nitric Oxide Synthase Type III Physiology Adipose tissue Vasodilation 030204 cardiovascular system & hematology Mice 03 medical and health sciences 0302 clinical medicine Adipokines Superoxides Enos Physiology (medical) Internal medicine medicine Animals Obesity Phenylephrine Protein kinase B Aorta Pharmacology biology Tumor Necrosis Factor-alpha Chemistry Adenylate Kinase AMPK biology.organism_classification Diet Mice Inbred C57BL 030104 developmental biology Endocrinology Adipose Tissue Gene Expression Regulation Vasoconstriction Tumor necrosis factor alpha Diet-induced obese Signal Transduction medicine.drug |
| Zdroj: | Clinical and Experimental Pharmacology and Physiology. 45:50-57 |
| ISSN: | 0305-1870 |
| DOI: | 10.1111/1440-1681.12851 |
| Popis: | The anti-contractile property of perivascular adipose tissue (PVAT) is abolished through an endothelium-dependent pathway in obesity. C1q/tumor necrosis factor-related protein (CTRP)9 improved endothelial function by promoting endothelium-dependent vasodilatation. The aims of this study were to investigate whether CTRP9 improves the anti-contractile effect of PVAT and protects against PVAT dysfunction in obese mice. The mice were treated with a high-fat diet with or without CTRP9 treatment. Thoracic aortas with or without PVAT (PVAT+ or PVAT-) were prepared, and concentration-dependent responses to phenylephrine were measured. Obese mice showed a significantly increased contractile response, which was suppressed by CTRP9 treatment both with and without PVAT. PVAT significantly reduced the anti-contractile effect in obese mice, which was partially restored by CTRP9 treatment. Treatment of the aortic rings (PVAT+) with inhibitors of AMP protein kinase (AMPK), Akt and endothelial nitric oxide synthase (eNOS) attenuated the beneficial effect of CTRP9 on PVAT. Similar results were observed when we pretreated the aortic rings with CTRP9 ex vivo. CTRP9 significantly enhanced the phosphorylation levels of AMPK, Akt and eNOS, and reduced superoxide production and TNF-α levels in PVAT from obese mice. Our study suggests that CTRP9 enhanced the anti-contractile effect of PVAT and improved PVAT function by activating the AMPK-eNOS pathway in obese mice. |
| Databáze: | OpenAIRE |
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