Opisthorchis viverrini antigens up-regulates the expression of CD80 and MHC class II in JAWSII mouse dendritic cells and promotes IL-10 and TGF-β secretions
| Autor: | Suphattra Jittimanee, Surasakdi Wongratanacheewin, Jutharat Jittimanee, Chamraj Kaewraemruaen |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Genes MHC Class II chemical and pharmacologic phenomena Major histocompatibility complex Opisthorchiasis Mice Immune system Antigen Transforming Growth Factor beta Animals CD86 MHC class II Cluster of differentiation biology Chemistry Opisthorchis hemic and immune systems Dendritic Cells Interleukin-10 Up-Regulation Cell biology Mice Inbred C57BL Interleukin 10 Infectious Diseases Gene Expression Regulation Antigens Helminth B7-1 Antigen biology.protein Parasitology CD80 |
| Zdroj: | Parasitology International. 84:102401 |
| ISSN: | 1383-5769 |
| DOI: | 10.1016/j.parint.2021.102401 |
| Popis: | Dendritic cells (DCs) are antigen-presenting cells (APC) involved in the initiation of immune responses. Maturation of DCs is characterized by the high expression of major histocompatibility complex (MHC) class II and co-stimulatory clusters of differentiation (CD) 40, CD80, and CD86 molecules. Matured DCs are required for T cell differentiation and proliferation. However, the response of DCs to Opisthorchis viverrini antigens has not yet been understood. Therefore, this study sought to determine the expression of surface molecules of JAWSII mouse DCs stimulated by crude somatic (CS) and excretory-secretory (ES) antigens of O. viverrini. ES antigen significantly induced only mRNA expression of CD80 and MHC class II in JAWSII mouse DCs, while CS antigen promoted up-regulation of both mRNA and protein levels of CD80 and MHC class II, indicating relative maturation of JAWII mouse DCs. Moreover, the secreted cytokines from the co-cultures of O. viverrini antigens stimulated JAWSII DC with naive CD4+ T cells was determined. Significantly increased levels of immunosuppressive cytokines interleukin (IL)-10 and transforming growth factor beta (TGF-β) were found. The up-regulation of these cytokines may indicate the response of regulatory T cells (Treg) to CS antigen-stimulated JAWSII DC. These findings may lead to a better understanding of the role that DCs play in O. viverrini infection. |
| Databáze: | OpenAIRE |
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