Determination of berberine and the study of fluorescence quenching mechanism between berberine and enzyme-catalyzed product
| Autor: | Ningning Yue, Huai You Wang, Qingluan Lv, Bin Gong, Miao Zhang |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Berberine
Calibration curve Analytical chemistry Derivative Sensitivity and Specificity Endothermic process Armoracia Analytical Chemistry Ion chemistry.chemical_compound Hydrogen peroxide Instrumentation Horseradish Peroxidase Spectroscopy Plant Proteins Detection limit Binding Sites Hydrogen Peroxide Hydrogen-Ion Concentration Binding constant Atomic and Molecular Physics and Optics Spectrometry Fluorescence chemistry Calibration Thermodynamics |
| Zdroj: | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 73:682-686 |
| ISSN: | 1386-1425 |
| DOI: | 10.1016/j.saa.2009.03.017 |
| Popis: | A new method for determining berberine has been established based on the principle of fluorescence quenching. The calibration curve was found to be linear between F(0)/F and the concentration of berberine with the range of 3.00-20.0 microg mL(-1). The detection limit was 0.51 microg mL(-1) and the relative standard derivative was 0.18%. Effects of pH, foreign ions and the optimization of variables on the determination of berberine have been examined. The mechanism of the fluorescence quenching has been discussed. The binding constant and the number of binding sites were 1.70x10(6) L mol(-1) and 1.14, respectively. The data, DeltaH = 42.71 kJ mol(-1), DeltaS = 264.3 J K(-1) mol(-1) and the mean value DeltaG = -39.65 kJ mol(-1) were estimated which showed that the reaction was spontaneous and endothermic. The main binding force was hydrophobic force because both DeltaH and DeltaS were positive. |
| Databáze: | OpenAIRE |
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