Exposure of βL-crystallin to oxidizing free radicals enhances its susceptibility to transglutaminase activity
| Autor: | Trevor F. Slater, Enrico Gravela, M. Seccia, K H Cheeseman, O. Brossa |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Free Radicals
Tissue transglutaminase Radical Guinea Pigs Oxidative phosphorylation Biochemistry chemistry.chemical_compound Superoxides Crystallin Lens Crystalline Hydroxides Putrescine Animals Sulfhydryl Compounds Molecular Biology Transglutaminases biology Hydroxyl Radical Tryptophan Substrate (chemistry) Cell Biology Crystallins eye diseases Peroxides Spectrometry Fluorescence Liver Hydroperoxyl chemistry biology.protein Cattle Electrophoresis Polyacrylamide Gel sense organs Polyamine Research Article |
| Zdroj: | Biochemical Journal. 274:869-873 |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj2740869 |
| Popis: | 1. The effects of free radicals on the capacity of beta L-crystallin to act as a substrate for the enzyme transglutaminase were investigated. 2. beta L-Crystallin was exposed to defined radical species that were generated radiolytically, and transglutaminase activity, using the modified protein as substrate, was subsequently measured by monitoring the incorporation of [14C]putrescine. 3. Exposure of beta L-crystallin to hydroxyl radicals, thymine peroxyl radicals and acetone peroxyl radicals at concentrations of up to 135 microM increased the capacity of the protein to incorporate putrescine. With higher concentrations of these radicals this capacity of beta L-crystallin to act as a transglutaminase substrate declined to control levels or lower. 4. Superoxide radicals were inactive in this regard; hydroperoxyl radicals were active only at high concentrations. 5. It has previously been suggested that changes in the crystallins that occur during aging and with cataract may be due to oxidative reactions and to transglutaminase activity. This study suggests that these phenomena may be considered together rather than separately. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|