A single-step isolation of useful antioxidant compounds from Ishige okamurae by using centrifugal partition chromatography
| Autor: | Ju-Young Ko, You-Jin Jeon, Hyung-Ho Kim, Hyun-Soo Kim, Ji-Hyeok Lee, Chul Young Kim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Antioxidant medicine.medical_treatment Electrospray ionization ABTS+ online HPLC Ethyl acetate Fraction (chemistry) Aquatic Science Oceanography Biochemistry High-performance liquid chromatography lcsh:Aquaculture. Fisheries. Angling 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology Octaphlorethol A medicine Ishige okamurae Centrifugal partition chromatography (CPC) Molecular Biology lcsh:SH1-691 ABTS Chromatography 04 agricultural and veterinary sciences 040401 food science Diphlorethohydroxycarmalol Partition coefficient 030104 developmental biology chemistry Animal ecology |
| Zdroj: | Fisheries and Aquatic Sciences, Vol 19, Iss 1, Pp 1-7 (2016) |
| ISSN: | 2234-1757 |
| DOI: | 10.1186/s41240-016-0023-y |
| Popis: | One of the main compounds in Ishige okamurae, diphlorethohydroxycarmalol (DPHC), is known to exhibit antiviral and anti-inflammatory effects. However, it has not been investigated extensively. In this study, preparative centrifugal partition chromatography (CPC) coupled with 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS+) online HPLC was employed for effectively separating considerable amounts of antioxidant compounds from marine algae. Two main antioxidant compounds, DPHC and octaphlorethol A (OPA), respectively, were confirmed and isolated from the ethyl acetate (EtOAc) fraction of I. okamurae by ABTS+ online HPLC and preparative CPC systems. The presence of DPHC and OPA was confirmed in the EtOAc fraction of I. okamurae by both liquid chromatography with diode array detection and electrospray ionization mass spectrometry (LC-DAD-ESI/MS) and ABTS+ online HPLC systems: DPHC (39 mg) and OPA (23 mg) were successfully isolated from I. okamurae (500 mg) with optimum solvent composition (0.5:10:4:6; n-hexane/EtOAc/MeOH/water, v/v) with corresponding partition coefficients (K) of 1.62 and 2.71, respectively, by preparative CPC. Hence, CPC coupled with ABTS+ online HPLC is convenient for the efficient and simple isolation of these antioxidant compounds from I. okamurae. |
| Databáze: | OpenAIRE |
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