Formation of new capillary-like tubes in a three-dimensional in vitro model (Aorta/Collagen Gel)
| Autor: | Masumi Akita, Katsuji Kaneko, Eiko Murata, Hans-Joachim Merker |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Cell type
Time Factors Endothelium Angiogenesis Neovascularization Physiologic Biology Mice Organ Culture Techniques Vasculogenesis medicine Animals Aorta Gap junction General Medicine Anatomy In vitro Capillaries Rats Endothelial stem cell Microscopy Electron medicine.anatomical_structure Biophysics Lamina densa Collagen Endothelium Vascular Gels Developmental Biology |
| Zdroj: | Annals of Anatomy - Anatomischer Anzeiger. 179:137-147 |
| ISSN: | 0940-9602 |
| DOI: | 10.1016/s0940-9602(97)80088-x |
| Popis: | Direct sprouting (angiogenesis) does not occur during the formation of capillary-like tubes in an aorta/ collagen gel in the in vitro model. However, emigration of cells which stretch, arrange themselves side by side, form contacts (unspecific, tight and gap junctions), develop a lumen and show differentiation of endothelial cells (including the formation of a lamina densa and the appearance of pericytes) have been observed, i.e. vasculogenesis occurs. The origin of long, stretched cells is not known with certainty. They possibly represent smooth muscle cells. In addition, other cell types have been found, such as fibrocyte-like and fibroblast-like cells, elastoblasts, fat cells, monocytes and macrophages. All these cells are able to produce factors that promote the formation of new capillaries. Hence, a knowledge of these cells appears to be important for the analysis of in vitro systems. Moreover, the occurrence of these cell types must be considered when assessing possible effects. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect