Effects of human Parvovirus B19 and Bocavirus VP1 unique region on tight junction of human airway epithelial A549 cells
Autor: | Tsai-Ching Hsu, Yuan-Chao Hsiao, Jiann-Jou Yang, Chun-Ching Chiu, Bor-Show Tzang, Ya-Fang Shi |
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Jazyk: | angličtina |
Rok vydání: | 2014 |
Předmět: |
Inflammatory Diseases
viruses Gene Expression lcsh:Medicine Respiratory Mucosa Phospholipase Occludin Cell Line Tight Junctions Phospholipase A2 Complementary and Alternative Medicine Human bocavirus Claudin-1 Electric Impedance Parvovirus B19 Human Medicine and Health Sciences medicine Humans lcsh:Science A549 cell Multidisciplinary biology Tight junction Tumor Necrosis Factor-alpha Viral Core Proteins lcsh:R virus diseases Epithelial Cells biology.organism_classification Molecular biology Recombinant Proteins Bee Venoms Phospholipases A2 Infectious Diseases medicine.anatomical_structure Cell culture Immunology biology.protein lcsh:Q Sodium-Potassium-Exchanging ATPase Research Article Respiratory tract |
Zdroj: | PLoS ONE, Vol 9, Iss 9, p e107970 (2014) PLoS ONE |
ISSN: | 1932-6203 |
Popis: | As is widely recognized, human parvovirus B19 (B19) and human bocavirus (HBoV) are important human pathogens. Obviously, both VP1 unique region (VP1u) of B19 and HBoV exhibit the secreted phospholipase A2 (sPLA2)-like enzymatic activity and are recognized to participate in the pathogenesis of lower respiratory tract illnesses. However, exactly how, both VP1u from B19 and HBoV affect tight junction has seldom been addressed. Therefore, this study investigates how B19-VP1u and HBoV-VP1u may affect the tight junction of the airway epithelial A549 cells by examining phospholipase A2 activity and transepithelial electrical resistance (TEER) as well as performing immunoblotting analyses. Experimental results indicate that TEER is more significantly decreased in A549 cells by treatment with TNF-α (10 ng), two dosages of B19-VP1u and BoV-VP1u (400 ng and 4000 ng) or bee venom PLA2 (10 ng) than that of the control. Accordingly, more significantly increased claudin-1 and decreased occludin are detected in A549 cells by treatment with TNF-α or both dosages of HBoV-VP1u than that of the control. Additionally, more significantly decreased Na+/K+ ATPase is observed in A549 cells by treatment with TNF-α, high dosage of B19-VP1u or both dosages of BoV-VP1u than that of the control. Above findings suggest that HBoV-VP1u rather than B19 VP1u likely plays more important roles in the disruption of tight junction in the airway tract. Meanwhile, this discrepancy appears not to be associated with the secreted phospholipase A2 (sPLA2)-like enzymatic activity. |
Databáze: | OpenAIRE |
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