Identification of Cofilin-1 and Destrin as Potential Early-warning Biomarkers for Gamma Radiation in Mouse Liver Tissues
| Autor: | Fanghui Xu, Lan Yi, Jing Sun, Hongxiang Mu, Nanyang Yang, Dexin Ding, Jie Yin, Nan Hu, Keren Dai |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Cofilin 1
Male Proteome Epidemiology Health Toxicology and Mutagenesis medicine.disease_cause 030218 nuclear medicine & medical imaging Mice 03 medical and health sciences 0302 clinical medicine Western blot Peptide mass fingerprinting medicine Animals Radiology Nuclear Medicine and imaging medicine.diagnostic_test Chemistry Cancer medicine.disease Fold change Mice Inbred C57BL Destrin Liver Gamma Rays Apoptosis 030220 oncology & carcinogenesis Cancer research Carcinogenesis Biomarkers |
| Zdroj: | Health Physics. 116:749-759 |
| ISSN: | 1538-5159 0017-9078 |
| DOI: | 10.1097/hp.0000000000001012 |
| Popis: | Gamma radiation causes cell injury and leads to an increased risk of cancer, so it is of practical significance to identify biomarkers for gamma radiation. We used proteomic analysis to identify differentially expressed proteins in liver tissues of C57BL/6J mice treated with gamma radiation from Cs for 360 d. We confirmed obvious pathological changes in mouse liver tissues after irradiation. Compared with the control group, 74 proteins showed a fold change of ≥1.5 in the irradiated groups. We selected 24 proteins for bioinformatics analysis and peptide mass fingerprinting and found that 20 of the identified proteins were meaningful. These proteins were associated with tumorigenesis, tumor suppression, catalysis, cell apoptosis, cytoskeleton, metabolism, gene transcription, T-cell response, and other pathways. We confirmed that both cofilin-1 and destrin were up regulated in the irradiated groups by western blot and real-time polymerase chain reaction. Our findings indicate that cofilin-1 and destrin are sensitive to gamma radiation and may be potential biomarkers for gamma radiation. Whether these proteins are involved in radiation-induced tumorigenesis requires further investigation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|