Active Delivery of Single DNA Molecules into a Plasmonic Nanopore for Label-Free Optical Sensing
| Autor: | Cees Dekker, Daniel Verschueren, Xin Shi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
single-molecule sensing
Materials science Letter Light Bioengineering 02 engineering and technology 010402 general chemistry 01 natural sciences Plasmon resonance sensing Diffusion Motion Nanopores Electric field Nanotechnology General Materials Science Surface plasmon resonance nanopore single-particle scattering Plasmon chemistry.chemical_classification business.industry Mechanical Engineering Biomolecule General Chemistry DNA Equipment Design Surface Plasmon Resonance 021001 nanoscience & nanotechnology Condensed Matter Physics 0104 chemical sciences Light intensity Nanopore plasmonic nanopore chemistry Temporal resolution Optoelectronics Gold 0210 nano-technology business Biosensor |
| Zdroj: | Nano Letters Nano Letters: a journal dedicated to nanoscience and nanotechnology, 18(12) |
| ISSN: | 1530-6992 1530-6984 |
| Popis: | Plasmon resonance biosensors provide ultimate sensitivity at the single-molecule level. This sensitivity is, however, associated with a nanometer-sized confined hotspot, and molecular transport toward the sensor relies on inefficient diffusion. Here, we combine a plasmonic nanoantenna with a solid-state nanopore and demonstrate that single DNA molecules can be efficiently delivered to the plasmonic hotspots and detected in a label-free manner at submillisecond acquisition rates by monitoring the backscattered light intensity from the plasmonic nanoantennas. Our method realizes a better than 200 μs temporal resolution together with a down to subsecond waiting time, which is orders of magnitude better than traditional single-molecule plasmonic resonance sensing methods. Furthermore, the electric field applied to the nanopore can actively drive biomolecules away from the hotspot, preventing molecules to permanently bind to the gold sensor surface and allowing efficient reuse of the sensor. Our plasmonic nanopore sensor thus significantly outperforms conventional plasmon resonance sensors and provides great opportunities for high-throughput optical single-molecule-sensing assays. |
| Databáze: | OpenAIRE |
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