Evaluation of sheep ovarian tissue cryopreservation with slow freezing or vitrification after chick embryo chorioallantoic membrane transplantation
| Autor: | Mahboubeh Vatanparast, Mehdi Mohsenzadeh, Marjan Omidi, Mohammad Ali Khalili, Nahid Yari |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Necrosis Chick Embryo Chorioallantoic Membrane General Biochemistry Genetics and Molecular Biology Cryopreservation Andrology 03 medical and health sciences 0302 clinical medicine Ovarian Follicle Freezing Follicular phase medicine Animals Vitrification Ovarian tissue cryopreservation Sheep 030219 obstetrics & reproductive medicine Chemistry Fertility Preservation General Medicine Embryo Transfer Transplantation Chorioallantoic membrane 030104 developmental biology Heterografts Female Folliculogenesis medicine.symptom General Agricultural and Biological Sciences Chickens |
| Zdroj: | Cryobiology. 81:178-184 |
| ISSN: | 0011-2240 |
| DOI: | 10.1016/j.cryobiol.2018.01.002 |
| Popis: | The aim of our investigations was to compare the effectiveness of two methods for cryopreservation of sheep ovarian tissue, slow freezing and vitrification. The quality of cryopreserved tissues was evaluated after 5 days of thawing and chorioallantoic membrane (CAM) transplantation. Follicular structure, stromal integrity and neovascularization were assessed. The areas of fibrosis and necrosis were measured using MICROVISIBLE software, and proliferation was assessed with Ki-67 immunostaning. After 5 days of culture, the proportion of primordial follicles decreased, whereas the primary and intermediary follicles increased insignificantly (p > .05). Only necrosis in the vitrified culture group increased significantly (p |
| Databáze: | OpenAIRE |
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