Sterol-dependent nuclear import of ORP1S promotes LXR regulated trans-activation of apoE
| Autor: | Sungsoo Lee, Yangsik Jeong, Peter Michaely, Ping-yuan Wang, David J. Mangelsdorf, Richard G.W. Anderson |
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| Rok vydání: | 2012 |
| Předmět: |
Transcriptional Activation
Receptors Steroid Transcription Genetic Molecular Sequence Data Active Transport Cell Nucleus Biology Transfection Article Apolipoproteins E Genes Reporter polycyclic compounds Humans Amino Acid Sequence Luciferases Liver X receptor Transcription factor Liver X Receptors Cell Biology Orphan Nuclear Receptors Sterol transport Sterol Sterol regulatory element-binding protein Kinetics Sterols Enhancer Elements Genetic HEK293 Cells Biochemistry lipids (amino acids peptides and proteins) Sterol binding Nuclear transport Oxysterol-binding protein HeLa Cells Protein Binding Signal Transduction |
| Zdroj: | Experimental Cell Research. 318:2128-2142 |
| ISSN: | 0014-4827 |
| Popis: | Oxysterol binding protein related protein 1S (ORP1S) is a member of a family of sterol transport proteins. Here we present evidence that ORP1S translocates from the cytoplasm to the nucleus in response to sterol binding. The sterols that best promote nuclear import of ORP1S also activate the liver X receptor (LXR) transcription factors and we show that ORP1S binds to LXRs, promotes binding of LXRs to LXR response elements (LXREs) and specifically enhances LXR-dependent transcription via the ME.1 and ME.2 enhancer elements of the apoE gene. We propose that ORP1S is a cytoplasmic sterol sensor, which transports sterols to the nucleus and promotes LXR-dependent gene transcription through select enhancer elements. |
| Databáze: | OpenAIRE |
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