Effect of acute and short-term dietary fat ingestion on postprandial skeletal muscle protein synthesis rates in middle-aged, overweight, and obese men
| Autor: | Francis B. Stephens, Amanda Davies, Kostas Tsintzas, Dong-Hyun Kim, Christopher J. Gaffney, David A. Barrett, Joanne Mallinson, Pardeep Pabla, Tariq Taylor, Robert G. Jones, Carolyn Chee, Scott Cooper, Luc J. C. van Loon |
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| Přispěvatelé: | Humane Biologie, RS: NUTRIM - R3 - Respiratory & Age-related Health |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
obesity Anabolism Physiology intramuscular lipids Endocrinology Diabetes and Metabolism Muscle Proteins Overweight OXIDATION Protein biosynthesis Ingestion ANABOLIC RESISTANCE Amino Acids GENE-EXPRESSION Cross-Over Studies GLUCOSE-METABOLISM Middle Aged Milk Proteins Postprandial medicine.anatomical_structure dietary fat medicine.symptom SENSITIVITY WHOLE-BODY medicine.medical_specialty skeletal muscle protein synthesis postprandial period EXERCISE Hyperphagia Insulin resistance LIPID-METABOLISM Physiology (medical) Internal medicine medicine Humans Muscle Skeletal business.industry Skeletal muscle medicine.disease Lipid Metabolism ACIDS Obesity Dietary Fats Kinetics Endocrinology Glucose Insulin Resistance INDUCED INSULIN-RESISTANCE business |
| Zdroj: | American Journal of Physiology : Endocrinology and Metabolism, 318(3), E417-E429. American Physiological Society |
| ISSN: | 0193-1849 1522-1555 |
| DOI: | 10.1152/ajpendo.00344.2019 |
| Popis: | Muscle anabolic resistance to dietary protein is associated with obesity and insulin resistance. However, the contribution of excess consumption of fat to anabolic resistance is not well studied. The aim of these studies was to test the hypothesis that acute and short-term dietary fat overload will impair the skeletal muscle protein synthetic response to dietary protein ingestion. Eight overweight/obese men [46.4 ± 1.4 yr, body mass index (BMI) 32.3 ± 5.4 kg/m2] participated in the acute feeding study, which consisted of two randomized crossover trials. On each occasion, subjects ingested an oral meal (with and without fat emulsion), 4 h before the coingestion of milk protein, intrinsically labeled with [1-13C]phenylalanine, and dextrose. Nine overweight/obese men (44.0 ± 1.7 yr, BMI 30.1 ± 1.1 kg/m2) participated in the chronic study, which consisted of a baseline, 1-wk isocaloric diet, followed by a 2-wk high-fat diet (+25% energy excess). Acutely, incorporation of dietary amino acids into the skeletal muscle was twofold higher ( P < 0.05) in the lipid trial compared with control. There was no effect of prior lipid ingestion on indices of insulin sensitivity (muscle glucose uptake, pyruvate dehydrogenase complex activity, and Akt phosphorylation) in response to the protein/dextrose drink. Fat overfeeding had no effect on muscle protein synthesis or glucose disposal in response to whey protein ingestion, despite increased muscle diacylglycerol C16:0 ( P = 0.06) and ceramide C16:0 ( P < 0.01) levels. Neither acute nor short-term dietary fat overload has a detrimental effect on the skeletal muscle protein synthetic response to dietary protein ingestion in overweight/obese men, suggesting that dietary-induced accumulation of intramuscular lipids per se is not associated with anabolic resistance. |
| Databáze: | OpenAIRE |
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