Determination of Barbiturates in Biological Specimens by Flat Membrane-Based Liquid-Phase Microextraction and Liquid Chromatography-Mass Spectrometry

Autor: Ying Dong, Chuixiu Huang, Ruiqin Zhu, Cai Xiangyang
Rok vydání: 2019
Předmět:
Male
Liquid Phase Microextraction
Pharmaceutical Science
02 engineering and technology
Mass spectrometry
liver
01 natural sciences
barbiturates
Article
Analytical Chemistry
lcsh:QD241-441
lcsh:Organic chemistry
Liquid chromatography–mass spectrometry
Tandem Mass Spectrometry
Drug Discovery
medicine
Animals
Sample preparation
simultaneous determination
Physical and Theoretical Chemistry
Whole blood
Detection limit
Chromatography
medicine.diagnostic_test
Chemistry
010401 analytical chemistry
Organic Chemistry
Extraction (chemistry)
whole blood
Reproducibility of Results
Repeatability
Hydrogen-Ion Concentration
021001 nanoscience & nanotechnology
urine
0104 chemical sciences
Rats
Chemistry (miscellaneous)
Therapeutic drug monitoring
Solvents
Molecular Medicine
membrane-based microextraction
0210 nano-technology
Chromatography
Liquid
Zdroj: Molecules
Molecules, Vol 24, Iss 8, p 1494 (2019)
Volume 24
Issue 8
ISSN: 1420-3049
Popis: The wide abuse of barbiturates has aroused extensive public concern. Therefore, the determination of such drugs is becoming essential in therapeutic drug monitoring and forensic science. Herein, a simple, efficient, and inexpensive sample preparation technique, namely, flat membrane-based liquid-phase microextraction (FM-LPME) followed by liquid chromatography-mass spectrometry (LC-MS), was used to determine barbiturates in biological specimens. Factors that may influence the efficiency including organic extraction solvent, pH, and composition of donor and acceptor phases, extraction time, and salt addition to the sample (donor phase) were investigated and optimized. Under the optimized extraction conditions, the linear ranges of the proposed FM-LPME/LC-MS method (with correlation coefficient factors &ge
0.99) were 7.5&ndash
750 ng mL&minus
1 for whole blood, 5.0&ndash
500 ng mL&minus
1 for urine, and 25&ndash
2500 ng g&minus
1 for liver. Repeatability between 5.0 and 13.7% was obtained and the limit of detection (LOD) values ranged from 1.5 to 3.1 ng mL&minus
1, from 0.6 to 3.6 ng mL&minus
1, and from 5.2 to 10.0 ng g&minus
1 for whole blood, urine, and liver samples, respectively. This method was successfully applied for the analysis of barbiturates in blood and liver from rats treated with these drugs, and excellent sample cleanup was achieved.
Databáze: OpenAIRE
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