Preinitiation complex assembly: potentially a bumpy path
| Autor: | Alexis R Kays, Julia M. Cox, Alanna Schepartz, Jennifer F. Sanchez |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
endocrine system
Transcription Genetic Chemistry genetic processes Kinetics DNA macromolecular substances biochemical phenomena metabolism and nutrition TATA-Box Binding Protein Cleavage (embryo) TATA Box Biochemistry Analytical Chemistry DNA-Binding Proteins DNA metabolism Crystallography Transcription preinitiation complex Biophysics Dna bending Humans Transcription Factors |
| Zdroj: | Current Opinion in Chemical Biology. 2:11-17 |
| ISSN: | 1367-5931 |
| DOI: | 10.1016/s1367-5931(98)80030-6 |
| Popis: | During 1996 and 1997, several chemical issues that arise in the early stages of preinitiation complex (PIC) formation were resolved. Kinetics experiments indicated that both TBP dimerization and DNA bending influence the rate of TBP-TATA box assembly. Affinity cleavage experiments indicated that TBP lacks the specificity to nucleate assembly of a properly oriented PIC. Finally, high-resolution structures provided the atomic detail of early intermediates in PIC formation. |
| Databáze: | OpenAIRE |
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