GLUT4 molecules are recruited at random for insertion within the plasma membrane upon insulin stimulation

Autor: Roland Govers, Yannick Le Marchand-Brustel, Marion Berenguer
Přispěvatelé: Centre méditerranéen de médecine moléculaire (C3M), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA), Nutrition, obésité et risque thrombotique (NORT), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Jazyk: angličtina
Rok vydání: 2010
Předmět:
endocrine system diseases
medicine.medical_treatment
Cell
Stimulation
[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
Biochemistry
MESH: Dose-Response Relationship
Drug
Mice
Structural Biology
Insulin
MESH: Animals
Bovine serum albumin
biology
musculoskeletal system
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biomolecules [q-bio.BM]
Dose–response relationship
Protein Transport
Membrane
medicine.anatomical_structure
hormones
hormone substitutes
and hormone antagonists
medicine.medical_specialty
MESH: Protein Transport
Protein trafficking
Biophysics
MESH: Insulin
Internal medicine
3T3-L1 Cells
MESH: Hypoglycemic Agents
Genetics
medicine
Animals
Hypoglycemic Agents
Glucose transporter type 4
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biochemistry [q-bio.BM]
Molecular Biology
MESH: Mice
Protein translocation
Dose-Response Relationship
Drug
Cell Membrane
Glucose transporter
nutritional and metabolic diseases
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
Cell Biology
MESH: 3T3-L1 Cells
Endocrinology
biology.protein
MESH: Glucose Transporter Type 4
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
GLUT4
Insulin-responsive glucose transporter
MESH: Cell Membrane
Zdroj: FEBS Letters
FEBS Letters, Wiley, 2010, 584 (3), pp.537-42
ISSN: 0014-5793
1873-3468
Popis: International audience; Glucose transporter 4 (GLUT4) is efficiently retained intracellularly. Here, we investigated the insulin-induced reduction of retention. While increasing insulin concentrations led to gradual increases in both the amount of recycling GLUT4 molecules and cell surface GLUT4 levels, the kinetics of the increase in time was independent of insulin concentration. To determine whether there are GLUT4 subpools that have a distinct insulin sensitivity, adipocytes were consecutively stimulated twice with a low concentration of insulin while recycling GLUT4 molecules were continuously labeled. This revealed that not the same pool of GLUT4 molecules was mobilized twice and thus that upon insulin stimulation, GLUT4 is likely to be recruited at random for insertion within the plasma membrane.
Databáze: OpenAIRE
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