Housekeeping proteins: How useful are they in skeletal muscle diabetes studies and muscle hypertrophy models?
| Autor: | Gabriel Nasri Marzuca-Nassr, Carlos Hermano da Justa Pinheiro, Rui Curi, Philip Newsholme, Kaio Fernando Vitzel, Marco Aurélio Salomão Fortes |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Blotting Western Biophysics 030204 cardiovascular system & hematology Biology Biochemistry Streptozocin Diabetes Mellitus Experimental Muscle hypertrophy 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine stomatognathic system Western blot WESTERN BLOTTING Tubulin Diabetes mellitus medicine Animals Ponceau S Actinin Rats Wistar Muscle Skeletal Molecular Biology Glyceraldehyde 3-phosphate dehydrogenase medicine.diagnostic_test Glyceraldehyde-3-Phosphate Dehydrogenases Skeletal muscle Cell Biology medicine.disease Actins Rats Staining 030104 developmental biology medicine.anatomical_structure chemistry Housekeeping Hyperglycemia biology.protein |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/j.ab.2016.03.023 |
| Popis: | The use of Western blot analysis is of great importance in research, and the measurement of housekeeping proteins is commonly used for loading controls. However, Ponceau S staining has been shown to be an alternative to analysis of housekeeping protein levels as loading controls in some conditions. In the current study, housekeeping protein levels were measured in skeletal muscle hypertrophy and streptozotocin-induced diabetes experimental models. The following housekeeping proteins were investigated: glyceraldehyde-3-phosphate dehydrogenase (GAPDH), β-actin, α-tubulin, γ-tubulin, and α-actinin. Evidence is presented that Ponceau S is more reliable than housekeeping protein levels for specific protein quantifications in Western blot analysis. |
| Databáze: | OpenAIRE |
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