Evodiamine alleviates kidney ischemia reperfusion injury in rats: A biochemical and histopathological study

Autor: Ayhan Tanyeli, Elif Polat, Zeliha Yetim, Ersen Eraslan
Rok vydání: 2019
Předmět:
Male
0301 basic medicine
Interleukin-1beta
Anti-Inflammatory Agents
Apoptosis
Pharmacology
Kidney
medicine.disease_cause
Biochemistry
Antioxidants
chemistry.chemical_compound
Renal Artery
0302 clinical medicine
chemistry.chemical_classification
Caspase 3
NF-kappa B
Acute Kidney Injury
Surgical Instruments
Interleukin-10
Treatment Outcome
medicine.anatomical_structure
Reperfusion Injury
030220 oncology & carcinogenesis
Ischemia
Proinflammatory cytokine
03 medical and health sciences
Evodiamine
medicine
Animals
Rats
Wistar
Molecular Biology
Inflammation
Reactive oxygen species
Renal ischemia
Interleukin-6
Tumor Necrosis Factor-alpha
business.industry
Cell Biology
medicine.disease
Rats
Oxidative Stress
030104 developmental biology
Gene Expression Regulation
chemistry
Quinazolines
Reactive Oxygen Species
business
Reperfusion injury
Oxidative stress
Zdroj: Journal of Cellular Biochemistry. 120:17159-17166
ISSN: 1097-4644
0730-2312
DOI: 10.1002/jcb.28976
Popis: Renal ischemia/reperfusion (I/R) injury resulting in acute renal failure, is a major clinical problem due to its high mortality rate. Renal I/R increases the reactive oxygen species, secretion of inflammatory cytokines, chemokines and other factors. This suggests that initiating the apoptosis process in the presence of oxidative stress may play a role in life-threatening conditions, such as ischemia. Ischemia reperfusion-induced renal damage can result in renal failure and death. Although many treatment procedures have been carried out to reduce or destroy renal I/R damage in experimental models, so far, a routine method of treatment has not yet been found. For this reason, the current study was planned to investigate the possible protective effects of evodiamine on tissue damage caused by ischemia-reperfusion in kidney tissue in rats and an experimental renal I/R model was used for this purpose. Four groups were formed in the study: the control, sham control, ischemia reperfusion (I/R), and evodiamine (10 mg/kg) + I/R groups. The effects of evodiamine against kidney I/R injury were investigated. TAS (total oxidant status), TOS (total oxidant status), interleukin-1β (IL-1β), IL-6, IL-10 and tumor necrosis factor-α levels were determined by enzyme-linked immunosorbent assay. The oxidative stress index was calculated from TAS and TOS levels. In addition, the renal ischemia reperfusion injury was examined histopathologically. The IL-10 and TAS levels in the I/R group decreased when compared with the control and Sham groups, while these levels increased in the evodiamine group. Histopathologic examination revealed that caspase 3 and nuclear factor-κB levels decreased in the evodiamine group compared with the I/R group. The application of evodiamine significantly reduced ischemia reperfusion-induced kidney damage due to its antioxidant, anti-inflammatory and antiapoptotic properties.
Databáze: OpenAIRE
Externí odkaz: