Acute Kidney Injury Induced by Pneumoperitoneum Pressure Via a Mitochondrial Injury-dependent Mechanism in a Rabbit Model of Different Degrees of Hydronephrosis
| Autor: | Rao Ting, Fan Cheng, Zhixiu Cao, Wei Li, Xiaobing Yao, Weimin Yu |
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| Rok vydání: | 2018 |
| Předmět: |
Male
medicine.medical_specialty Urology Blotting Western 030232 urology & nephrology Hydronephrosis Risk Assessment Sensitivity and Specificity Severity of Illness Index 03 medical and health sciences Random Allocation 0302 clinical medicine Pneumoperitoneum Western blot Pressure Medicine Animals Membrane Potential Mitochondrial Kidney medicine.diagnostic_test biology business.industry Caspase 3 Cytochrome c Acute kidney injury Insufflation Acute Kidney Injury medicine.disease Caspase 9 Disease Models Animal medicine.anatomical_structure Vacuolization Apoptosis 030220 oncology & carcinogenesis biology.protein Metalloproteases Laparoscopy Rabbits business Pneumoperitoneum Artificial |
| Zdroj: | Urology. 127 |
| ISSN: | 1527-9995 |
| Popis: | OBJECTIVE To clarify the effect of mitochondrial injury during laparoscopic surgery of the kidney in different degrees of hydronephrosis in rabbit model. METHODS A total of 90 rabbits were randomly allocated into 3 groups (groups PN, PM, and PS, ie, rabbits without, with mild and with severe hydronephrosis, respectively). The rabbits in the PM group (n = 30) and PS group (n = 30) underwent surgical procedures that induced mild and severe left hydronephrosis, respectively. The rabbits in all the groups were then allocated into 5 subgroups and were subjected to intra-abdominal pressures of 0, 6, 9, 12, and 15 mmHg. Changes in the mitochondrial membrane potential and mitochondrial electron microstructure were observed. The apoptosis proteins cytochrome C, apoptosis-inducing factor, caspase-3, and caspase-9 were measured by western blot analysis. RESULTS As the degrees of hydronephrosis increased, histopathological changes such as the decrease in mitochondrial membrane potential and mitochondrial vacuolization along with increased expression of apoptosis proteins, cytochrome C, apoptosis-inducing factor, caspase-3 gained statistically significance at lower intra-abdominal pressures (In PN and PM groups at 15 mmHg, and in PS group at 9 mmHg; for all P CONCLUSION Mitochondrial injury plays an important role during acute kidney injury induced by pneumoperitoneal pressure in different degrees of hydronephrosis in the rabbit model. |
| Databáze: | OpenAIRE |
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