Natural history of restricted synthesis and expression of measles virus genes in subacute sclerosing panencephalitis
| Autor: | Peter Ventura, Hubert E. Blum, Wallace W. Tourtellotte, Ashley T. Haase, David Walker, Stephen W. Wietgrefe, Mary Zupancic, B. Eble, D. Gantz, Linda Stowring |
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| Rok vydání: | 1985 |
| Předmět: |
Multidisciplinary
Slow virus Viral matrix protein Genes Viral biology viruses Brain Nucleic Acid Hybridization biology.organism_classification medicine.disease Measles Virology Virus Subacute sclerosing panencephalitis Measles virus Viral Proteins Viral replication Immunology medicine Humans RNA Viral Subacute Sclerosing Panencephalitis Gene Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 82:3020-3024 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.82.9.3020 |
| Popis: | Subacute sclerosing panencephalitis (SSPE) is a slow infection caused by measles virus in which several years separate recovery from typical acute measles and the development of a slowly progressive neurological disease. We have investigated replication of measles virus in brain tissue obtained after the onset of neurological disease and in the terminal phase. With a hybridization tomographic technique that combines in situ hybridization with macroradioautographic screening of large areas of tissue, we analyzed the spatial and temporal distribution of virus genes in vivo, using region- and strand-specific probes for the nucleocapsid and matrix genes. We show that early in the course of SSPE there is a global repression in the synthesis and expression of the genome. In the final stage of SSPE most infected cells still have depressed levels of plus- and minus-strand viral RNA and contain nucleocapsid protein but lack matrix protein. These findings provide further evidence for a unified view of slow infections of the nervous system, where the general constraints on virus gene expression provide an explanation for persistence of virus in the face of the host's immune response, and the slow evolution of pathological change. In the final phases of SSPE the more specific block in virus replication accounts for the cell-associated state of the virus and the difficulty in virus isolation. |
| Databáze: | OpenAIRE |
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