Photo-protective effect of sargachromenol against UVB radiation-induced damage through modulating cellular antioxidant systems and apoptosis in human keratinocytes
| Autor: | Mei Jing Piao, Hee Kyoung Kang, Sang Hee Byeon, Seung Ri Mun, Young Sang Koh, Pattage Madushan Dilhara Jayatissa Fernando, Nam Ho Lee, Eun Sook Yoo, Chang Sik Ko, Susara Ruwan Kumara Madduma Hewage, Mi Hee Ko, Jin Won Hyun |
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| Rok vydání: | 2016 |
| Předmět: |
Keratinocytes
0301 basic medicine MAPK/ERK pathway Ultraviolet Rays Health Toxicology and Mutagenesis Caspase 3 Toxicology medicine.disease_cause Superoxide dismutase 03 medical and health sciences 0302 clinical medicine medicine Humans Benzopyrans Extracellular Signal-Regulated MAP Kinases Pharmacology chemistry.chemical_classification Reactive oxygen species integumentary system biology Superoxide Dismutase Kinase Chemistry General Medicine Catalase Cytoprotection Caspase 9 Cell biology 030104 developmental biology Biochemistry 030220 oncology & carcinogenesis biology.protein Reactive Oxygen Species Sunscreening Agents Oxidative stress |
| Zdroj: | Environmental Toxicology and Pharmacology. 43:112-119 |
| ISSN: | 1382-6689 |
| Popis: | The aim of this study was to evaluate the photo-preventive effects of sargachromenol (SC) against ultraviolet B (UVB)-induced oxidative stress in human keratinocytes via assessing the antioxidant properties and underlying molecular mechanisms. SC exhibited a significant scavenging effect on UVB-induced intracellular reactive oxygen species (ROS). SC attenuated UVB-induced oxidative macromolecular damage, including the protein carbonyl content, DNA strand break, and 8-isoprostane level. Furthermore, SC decreased UVB-induced Bax, cleaved caspase-9, and cleaved caspase-3 protein levels, but increased that of Bcl-2, which are well-known key mediators of apoptosis. Moreover, SC increased superoxide dismutase, catalase, and heme oxygenase-1 protein expression. Pre-treatment with SC upregulated the main transcription factor of antioxidant enzymes, erythroid 2-related factor 2 level, which was reduced by UVB irradiation. Extracellular signal-regulated kinase (ERK) and Jun N-terminal kinases (JNK) are involved in the regulation of many cellular events, including apoptosis. SC treatment reversed ERK and JNK activation induced by UVB. Collectively, these data indicate that SC can provide remarkable cytoprotection against the adverse effects of UVB radiation by modulating cellular antioxidant systems, and suggest the potential of developing a medical agent for ROS-induced skin diseases. |
| Databáze: | OpenAIRE |
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