Preventing Pseudomonas aeruginosa Biofilms on Indwelling Catheters by Surface-Bound Enzymes
| Autor: | P. Lynne Howell, Hiram Sanchez, Dalal Asker, Benjamin Hatton, Ira Lacdao, Donald C. Sheppard, Stephanie Gilbert, Piyanka Sivarajah, Tarek S. Awad, Deepa Raju, David R. Andes |
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| Rok vydání: | 2021 |
| Předmět: |
Glycoside Hydrolases
Biomedical Engineering medicine.disease_cause Article Microbiology Biomaterials 03 medical and health sciences Catheters Indwelling Animals Central Venous Catheters Medicine 030304 developmental biology chemistry.chemical_classification 0303 health sciences 030306 microbiology business.industry Pseudomonas aeruginosa Biochemistry (medical) Biofilm General Chemistry Indwelling catheters Rats 3. Good health Enzyme chemistry Biofilms business |
| Zdroj: | ACS Appl Bio Mater |
| ISSN: | 2576-6422 |
| DOI: | 10.1021/acsabm.1c00794 |
| Popis: | Implanted medical devices such as central venous catheters are highly susceptible to microbial colonization and biofilm formation, and are a major risk factor for nosocomial infections. The opportunistic pathogen Pseudomonas aeruginosa uses exopolysaccharides, such as Psl, for both initial surface attachment and biofilm formation. We have previously shown that chemically immobilizing the Psl-specific glycoside hydrolase, PslG(h), to a material surface can inhibit P. aeruginosa biofilm formation. Herein we show that PslG(h) can be uniformly immobilized on the lumen surface of medical-grade, commercial polyethylene, polyurethane, and polydimethylsiloxane (silicone) catheter tubing. We confirmed the surface-bound PslG(h) was uniformly distributed along the catheter length and remained active even after storage for 30 days at 4 °C. P. aeruginosa colonization and biofilm formation under dynamic flow culture conditions in vitro showed a 3-log reduction in the number of bacteria during the first 11 days, and a 2-log reduction by day 14 for PslG(h) modified PE-100 catheters, compared to untreated catheter controls. In an in vivo rat infection model, PslG(h)-modified PE-100 catheters showed ~1.5-log reduction in the colonization of the clinical P. aeruginosa ATCC 27853 strain after 24 hours. These results demonstrate the robust ability of surface-bound glycoside hydrolase enzymes to inhibit biofilm formation, and their potential to reduce rates of device-associated infections. |
| Databáze: | OpenAIRE |
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