Carboxymethyl Cellulose Stimulates Rabbit Corneal Epithelial Wound Healing

Autor:	Joseph G. Vehige, Judith Flanagan, Mark D. P. Willcox, Qian Garrett, Zhenjun Zhao, Ashok Kumar, Peter A. Simmons, Ruo Zhong Xie, Shunjiang Xu
Rok vydání:	2008
Předmět:	medicine.medical_specialty Administration Topical Fluorophotometry Tight Junctions Immunoenzyme Techniques Cellular and Molecular Neuroscience Eye Injuries Ophthalmology Animals Medicine Wound Healing Dose-Response Relationship Drug integumentary system business.industry Epithelium Corneal technology industry and agriculture Membrane Proteins Phosphoproteins eye diseases Sensory Systems In vitro Epithelium Carboxymethyl cellulose Surgery Contact lens Disease Models Animal Dose–response relationship medicine.anatomical_structure Carboxymethylcellulose Sodium Zonula Occludens-1 Protein Immunohistochemistry Rabbits sense organs Ophthalmic Solutions business Wound healing medicine.drug
Zdroj:	Current Eye Research. 33:567-573
ISSN:	1460-2202 0271-3683
Popis:	Previously, we reported carboxymethyl cellulose (CMC) binding to human corneal epithelial cells and promoting corneal epithelial wound closure in vitro. Using an animal model, the efficacy of CMC in promoting corneal wound healing was examined.Following corneal epithelial wounding of NZ white rabbits, CMC (0.2% or 1.0%) or control vehicle (PBS) was administered topically (4 times daily for 3 days) to wounded and unwounded eyes with or without contact lens wear. Wound healing in response to the treatments was measured as percentage reduction of fluorescein-stained wound area 0 to 72 hr post-wounding. Corneas were examined histologically and expression of zonula occludens-1 (ZO-1) tight-junction was detected by immunohistochemistry.Percentage wound reduction in CMC-treated groups was significantly greater than controls (p0.05) at 24 and 32 hr. Complete wound closure was observed by 48 hr in 100% of CMC-treated eyes compared to 45% of vehicle-treated eyes. CMC also promoted wound closure dose-dependently. Epithelial cells formed an intact layer following CMC-treatment whereas vehicle-treated cells were less ordered. Strong ZO-1 expression in corneal epithelia of CMC-treated eyes was observed at 72 hr. Contact lens wear appeared to delay wound closure compared to without lens wear during CMC-treatment (p = 0.001).CMC promoted dose-dependent corneal epithelial wound healing. CMC stimulated ZO-1 expression, indicating accelerated corneal epithelial resistance barrier regeneration.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c91b082554ffaf3204eb4b6af55fcc8 https://doi.org/10.1080/02713680802140213 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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