Inducible synthesis of the Mitomycin C resistance gene product (MCRA) from Streptomyces lavendulae
| Autor: | John A. Rahn, Michael C. Flickinger, David H. Sherman, Paul R. August |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Mitomycin
Drug Resistance Locus (genetics) Biology Mitomycins Gene product chemistry.chemical_compound Bacterial Proteins Western blot Genetics medicine Inducer DNA Fungal chemistry.chemical_classification Antibiotics Antineoplastic medicine.diagnostic_test Mitomycin C General Medicine Molecular biology Streptomyces Enzyme chemistry Streptomyces lavendulae Oxidoreductases DNA DNA Damage |
| Zdroj: | Gene. 175:261-267 |
| ISSN: | 0378-1119 |
| Popis: | The mcr locus from Streptomyces lavendulae confers high level resistance (> 100 μg/ml) to mitomycin C (MC) and related mitomycins when cloned into Streptomyces lividans . Production of the mcrA gene product (MCRA) was shown to be MC-inducible by identification of MCRA ( M r of 54 kDa) using Western blot analysis and enzyme linked immunosorbent assay (ELISA). The magnitude of MCRA production was dependent on MC concentration, with primary induction starting at 0.1 lμg/ml and maximum induction at 10 μg/ml of the drug. Different levels of MCRA production were observed when other mitomycin metabolites were used as inducers, and the level of induction related directly to aziridine ring substitution on the individual molecules. Moreover, inducible synthesis of the mcrA gene product was unique to this structural class since production of MCRA did not occur as a general response to DNA damaging agents. The time profile of intracellular MCRA synthesis correlated with MC production in S. lavendulae , suggesting coordinated regulation of MC resistance and biosynthetic genes. |
| Databáze: | OpenAIRE |
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