B Cells of HIV-1–Infected Patients Bind Virions through Cd21–Complement Interactions and Transmit Infectious Virus to Activated T Cells
| Autor: | Tomeka Lowe, Anthony S. Fauci, Shuying Liu, Angela Malaspina, Jo Ann M. Mican, Tae-Wook Chun, Yuexia Li, Michael Baseler, Richard T. Davey, Susan Moir, Joseph W. Adelsberger, Linda A. Ehler |
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| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
viral reservoir
T-Lymphocytes Immunology B-cell receptor Antigen presentation Biology Lymphocyte Activation Interleukin 21 Immunology and Allergy Cytotoxic T cell CD21 Humans complement Antigen-presenting cell B cell Acquired Immunodeficiency Syndrome B-Lymphocytes CD40 Virion Antibodies Monoclonal Complement C3 Virology B-1 cell Chronic Disease Interleukin 12 biology.protein HIV-1 RNA Viral Original Article Receptors Complement 3d |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| Popis: | The impact of HIV-associated immunopathogenesis on B cells has been largely associated with indirect consequences of viral replication. This study demonstrates that HIV interacts directly with B cells in both lymphoid tissues and peripheral blood. B cells isolated from lymph node and peripheral blood mononuclear cells (PBMCs) of 4 and 23 chronically infected patients, respectively, demonstrated similar capacities to pass virus to activated HIV-negative PBMCs when compared with CD4+ cells from the same patients. However, in contrast to T cells, virus associated with B cells was surface bound, as shown by its sensitivity to pronase and the staining pattern revealed by in situ amplification of HIV-1 RNA. Cell sorting and ligand displacing approaches established that CD21 was the HIV-binding receptor on B cells, and that this association was mediated through complement-opsonized virus. These B cells were also found to express significantly lower levels of CD21 compared with HIV-negative individuals, suggesting a direct perturbing effect of HIV on B cells. These findings suggest that B cells, although they themselves are not readily infected by HIV, are similar to follicular dendritic cells in their capacity to serve as extracellular reservoirs for HIV-1. Furthermore, B cells possess the added capability of circulating in peripheral blood and migrating through tissues where they can potentially interact with and pass virus to T cells. |
| Databáze: | OpenAIRE |
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