Regulation of Type I Collagen Gene Expression in Bone
| Autor: | David W. Rowe, Alexander C. Lichtler, Paul H. Krebsbach, C.O. Woody, John R. Harrison, Antonio Bedalov, Dubravko Pavlin, Barbara E. Kream, Zoran Bogdanovic, Stephen H. Clark |
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| Rok vydání: | 1995 |
| Předmět: |
musculoskeletal diseases
Genetically modified mouse Transgene Parathyroid hormone Mice Transgenic Biology Biochemistry Bone and Bones Collagen Type I Fusion gene Mice Rheumatology Gene expression Animals Humans Orthopedics and Sports Medicine Vitamin D Promoter Regions Genetic Molecular Biology Cells Cultured Regulation of gene expression Bone Development Osteoblasts Skull Gene Expression Regulation Developmental Cell Biology Transfection Molecular biology Artificial Gene Fusion Rats Collagen Type I alpha 1 Chain Parathyroid Hormone Models Animal Type I collagen Interleukin-1 |
| Zdroj: | Connective Tissue Research. 31:261-264 |
| ISSN: | 1607-8438 0300-8207 |
| DOI: | 10.3109/03008209509010819 |
| Popis: | The regulation of COL1A1 gene expression in bone was studied by measuring the activity of type I collagen promoter fusion genes (ColCAT) in permanently transfected osteoblastic cells and calvariae from transgenic animals. The basal activity of ColCAT fusion genes in transfected cells is mediated by DNA sequences between -3.5 to -2.3 kb while expression in vivo requires sequences between -2.3 and -1.7 kb. Parathyroid hormone, 1,25-dihydroxyvitamin D3 and interleukin-1 decrease the activity of ColCAT fusion genes in osteoblastic cells and transgenic calvariae. Because there may be differences between the expression of ColCAT fusion genes in cultured cells and intact bone, it will be important to compare data obtained from transfected cells with an in vivo model such as calvariae from transgenic mice. |
| Databáze: | OpenAIRE |
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