Decreased susceptibility to chlorhexidine and distribution of qacA/B genes among coagulase-negative Staphylococcus clinical samples
| Autor: | Maria Carolina Soares Lopes, Bruna Costa Moura do Vale, Thiago André Cidral, Maria Celeste Nunes de Melo, Acácia Gentil Nogueira |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Coagulase Meticillin Staphylococcus 030106 microbiology Drug resistance Microbial Sensitivity Tests Biology Agar dilution Microbiology lcsh:Infectious and parasitic diseases 03 medical and health sciences Minimum inhibitory concentration 0302 clinical medicine Bacterial Proteins Drug Resistance Bacterial medicine Humans lcsh:RC109-216 030212 general & internal medicine Healthcare-associated infection Antiinfective agent Cross Infection Chlorhexidine Coagulase-negative Staphylococci Staphylococcal Infections Multiple drug resistance Infectious Diseases Methicillin Resistance qacA/B genes Brazil medicine.drug Research Article Disinfectants |
| Zdroj: | BMC Infectious Diseases BMC Infectious Diseases, Vol 19, Iss 1, Pp 1-5 (2019) |
| ISSN: | 1471-2334 |
| Popis: | Background Healthcare-associated infection (HAI) is a major public health problem. As a form of prevention and control, preparations of chlorhexidine are used extensively; however, the reduction of susceptibility to chlorhexidine has been reported. The aim of this study was to investigate the susceptibility to chlorhexidine and the distribution of the qacA/B genes in 211 clinical isolates of coagulase-negative Staphylococci (CoNS). Methods CoNS were identified by conventional biochemical tests. Antimicrobial susceptibility was tested by disk-diffusion. Minimum inhibitory concentration (MIC) of chlorhexidine was determined by agar dilution test; detection of the qacA/B and mecA genes were evaluated by PCR. Results The most frequently isolated species were S. epidermidis, S. hominis hominis, S. auricularis, and S. haemolyticus, respectively. The strains presented a multidrug resistance profile of 87%, including methicillin resistance. Reduced susceptibility to chlorhexidine was observed in 31%. The qacA/B genes were detected in samples resistant (32/32) and susceptible (17/32) to chlorhexidine. The vast majority (94%) of the samples with reduced susceptibility to chlorhexidine were multidrug resistant. Conclusions Our results show that qacA/B genes are not restricted to strains expressing chlorhexidine resistance. Further studies are needed to understand how the expression of these genes occurs. |
| Databáze: | OpenAIRE |
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