Levels of 8-hydroxy-2′-deoxyguanosine in cellular DNA from 12 tissues of young and old Sprague–Dawley rats
| Autor: | I. Schmerold, Hans Niedermüller |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
Aging medicine.medical_specialty DNA Repair DNA repair DNA damage Spleen Context (language use) Biology Kidney DNA Mitochondrial Biochemistry Rats Sprague-Dawley chemistry.chemical_compound Endocrinology Species Specificity Internal medicine Genetics medicine Animals Deoxyguanosine Tissue Distribution Molecular Biology Cell Nucleus 8-Hydroxy-2'-deoxyguanosine Kidney metabolism DNA Cell Biology DNA extraction Rats medicine.anatomical_structure Liver chemistry 8-Hydroxy-2'-Deoxyguanosine Oxidation-Reduction DNA Damage |
| Zdroj: | Experimental Gerontology. 36:1375-1386 |
| ISSN: | 0531-5565 |
| Popis: | The age dependent increase of 8-hydroxy-2'-deoxyguanosine (8-OH-dG) reported in DNA of organs of old rats appears to vary with the strain, age and sex of the animals used for the investigation. Here we report on 8-OH-dG concentrations in the cellular DNA of 12 tissues of male Sprague-Dawley rats aged 5 or 30 months and kept under standard conditions throughout their lives until being killed. DNA from frozen organs was isolated using a Qiagen DNA purification kit. Following digestion (nuclease P1, alkaline nuclease) hydrolysed DNA was applied onto a HPLC column; native nucleosides were monitored at 254 nm and 8-OH-dG by electrochemical detection. 8-OH-dG levels in organs of young rats ranged between 10 and 90 micromol/mol deoxyguanosine (dG). Highest levels (micromol 8-OH-dG /mol dG) were detected in the oesophagus (90), muscle (61), brain (65), liver (59), spleen (57), and testicles (63). 8-OH-dG in DNA from kidney, lung, heart, small and large intestine ranged between 28 and 38 micromol/mol dG. Lowest amounts were found in the glandular stomach (10). DNA of old rats generally contained higher 8-OH-dG levels with significant increases in liver (186%) and kidney (372%); other organs showed no significant decreases (spleen, brain, testicles) or increases up to 69% (heart). These findings are discussed in the context with previously published data on 8-OH-dG levels in organs from young and old rats. |
| Databáze: | OpenAIRE |
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