Interleukin 6 stimulates growth of vascular smooth muscle cells in a PDGF-dependent manner
| Autor: | S. Kano, Uichi Ikeda, A. Oguchi, M. Ikeda, T. Kamitani, T. Oohara, Yoshio Tsuruya |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
medicine.medical_specialty
Platelet-derived growth factor Vascular smooth muscle Physiology Antibodies Muscle Smooth Vascular chemistry.chemical_compound Physiology (medical) Internal medicine medicine Animals RNA Messenger Interleukin 6 Platelet-Derived Growth Factor Calcium metabolism Staining and Labeling DNA synthesis biology Interleukin-6 Osmolar Concentration musculoskeletal system Immunohistochemistry Endocrinology medicine.anatomical_structure chemistry Cell culture cardiovascular system biology.protein Calcium Cardiology and Cardiovascular Medicine tissues Cell Division Platelet-derived growth factor receptor Blood vessel |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 260:H1713-H1717 |
| ISSN: | 1522-1539 0363-6135 |
| DOI: | 10.1152/ajpheart.1991.260.5.h1713 |
| Popis: | We have investigated the effect of interleukin 6 (IL-6) on the growth of vascular smooth muscle cells (VSMC) isolated from rat aortas. Murine recombinant IL-6 significantly increased the number of VSMC and stimulated tritiated thymidine incorporation into VSMC in a dose-dependent manner. The IL-6-induced thymidine incorporation into VSMC was totally inhibited by the Ca2+ channel blocker verapamil; however, IL-6 showed no effects on the intracellular Ca2+ level ([Ca2+]i) in VSMC. Antibody against platelet-derived growth factor (PDGF) also totally inhibited the IL-6-induced thymidine uptake. PDGF caused a significant increase in the [Ca2+]i, which was totally inhibited by verapamil. IL-6 mRNA was not detected in unstimulated “quiescent” VSMC, but its expression was stimulated by exposure of VSMC to 10% fetal bovine serum. Immunohistochemical study using anti-PDGF antibody showed that IL-6 stimulated PDGF production in VSMC. These results support the premise that IL-6 is released by VSMC in an autocrine manner and promotes the growth of VSMC via induction of endogenous PDGF production. |
| Databáze: | OpenAIRE |
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