Induction of Tumor Necrosis Factor α Expression in Human T Lymphocytes Following Ionizing Gamma Irradiation
| Autor: | DOMINIQUE WEILL, FRANÇOISE GAY, MICHAEL G. TOVEY, SALEM CHOUAIB |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Messenger RNA
Base Sequence biology Tumor Necrosis Factor-alpha T-Lymphocytes Molecular Sequence Data Immunology Glyceraldehyde-3-Phosphate Dehydrogenases Dehydrogenase Cell Biology Molecular biology Ionizing radiation Secretory protein Gamma Rays Reference Values Virology biology.protein Humans Tumor necrosis factor alpha RNA Messenger Irradiation Cells Cultured Glyceraldehyde 3-phosphate dehydrogenase Gamma irradiation |
| Zdroj: | Journal of Interferon & Cytokine Research. 16:395-402 |
| ISSN: | 1557-7465 1079-9907 |
| DOI: | 10.1089/jir.1996.16.395 |
| Popis: | In this work, we present evidence that enriched human peripheral blood T lymphocytes, depleted of contaminating monocytes, rapidly express tumor necrosis factor alpha (TNF-alpha) mRNA when exposed to low doses of gamma-irradiation. In total PBL, TNF-alpha mRNA accumulation increased threefold as early as 30 minutes following exposure to 4 Gy and then declined to the baseline level by 3-5 h, as measured by the reverse transcriptase-polymerase chain reaction (RT-PCR). The increase in TNF-alpha mRNA was also observed in populations of enriched T cells and decreased when the dose of irradiation was increased to 10 Gy, strongly suggesting that T lymphocytes, the most radiosensitive cells of the body, contributed directly to the increase of TNF-alpha mRNA. A good correlation was found between mRNA expression and TNF-alpha protein secretion. Interestingly, a eightfold increase in glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA accumulation was also detected in both PBL and enriched T cells irradiated at 4 Gy for 3 h compared with unirradiated cells. This irradiation effect was almost completely abolished, however, following exposure to 10 Gy. Together these data suggest that T cells are responsible for the irradiation-induced expression of TNF-alpha and GAPDH. |
| Databáze: | OpenAIRE |
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