Spectrum of bacteriocin activity of Lactobacillus plantarum BS and fingerprinting by RAPD-PCR
| Autor: | Marcelina B. Lirazan, Helen A Mendoza, Francisco B. Elegado, Marie Antonette Ruth V Guerra, Rommel A Macayan |
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| Rok vydání: | 2003 |
| Předmět: |
Bacilli
Staphylococcus aureus medicine.disease_cause Escherichia coli O157 Microbiology fluids and secretions Bacteriocin Bacteriocins Species Specificity medicine Escherichia coli Phylogeny biology food and beverages General Medicine 16S ribosomal RNA biology.organism_classification DNA Fingerprinting RAPD Random Amplified Polymorphic DNA Technique Lactobacillus DNA profiling Listeria bacteria Lactobacillus plantarum Food Science |
| Zdroj: | International journal of food microbiology. 95(1) |
| ISSN: | 0168-1605 |
| Popis: | The spectrum of antimicrobial activity of Lactobacillus plantarum BS against representative bacterial species was established through deferred assay and 'spot-on-lawn' assay using actively growing cells and partially purified bacteriocin extract, respectively. Only lactobacilli, pediococci, enterococci, bacilli and Listeria were inhibited from the test microorganisms. Slight bacteriocinogenic activity through 'spot-on-lawn' assay was detected against Staphylococcus aureus and Escherichia coli O157:H7. Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) analysis was used to compare the fingerprint of L. plantarum BS with other strains of L. plantarum. Using the 16S rRNA-based primer, P32, the bacteriocinogenic isolate exhibited identical RAPD-PCR fingerprints to L. plantarum ATCC 14917. Dendrograms derived from the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) were constructed to show the similarity relationships among the investigated strains based on RAPD-PCR analysis. Bands differentiating L. plantarum BS from L. plantarum ATCC 14917 were also identified by varying the annealing temperature. |
| Databáze: | OpenAIRE |
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