Double Strandedness of Ribonucleic Acid of Bovine Ephemeral Fever Virus
| Autor: | Kunihiko Sato, Yuji Inaba, Tsuneyoshi Omori, Minoru Matumoto, Y. Ito, Yoshio Tanaka |
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| Rok vydání: | 1972 |
| Předmět: |
Guanine
Hot Temperature Fever RNase P viruses Cattle Diseases Cytidine Lithium Virus Phosphates law.invention Ribonucleases law Bovine ephemeral fever Centrifugation Density Gradient Animals Equilibrium Centrifugation Uracil Uridine Cells Cultured Infectivity biology Nucleotides Adenine RNA General Medicine biology.organism_classification Molecular biology Negative stain Microscopy Electron Biochemistry RNA Viral Viruses Unclassified Cattle Electron microscope |
| Zdroj: | Japanese Journal of Microbiology. 16:95-101 |
| ISSN: | 0021-5139 |
| DOI: | 10.1111/j.1348-0421.1972.tb00634.x |
| Popis: | Virus labeled with 3H-uridine or 32P-orthophosphate was purified by CsCl equilibrium centrifugation of concentrated virus materials from infectious BHK21-WI2 cell culture fluids. A single clearly visible band formed in the gradient coinciding with a sharp peak of radioactivity having a buoyant density of 1.19 g/ml. Infectivity exhibited a broader distribution with a peak coinciding with the visible band, in which numerous virions of the virus were observed with the aid of an electron microscope using the phosphotungstic negative staining technique. Ribonucleic acid (RNA) extracted from the purified virus by the phenol method exhibited a rather broad distribution of radioactivity with a major peak at about 12 S when analyzed by the sucrose density gradient centrifugation technique. Viral RNA centrifuged in the gradient after ribonuclcase (RNase) treatment showed a single sharp peak at about 12 S. These findings seemed to indicate that the virion of this virus contained double-stranded RNA. Double strandedness of the viral RNA was further corroborated by an examination of the base composition, reduced resistance to RNase in low salt concentration and a sharp thermal transition with a relatively high melting temperature of 85 C. The virus could not be classified either in the rhabdovirus group, although the shape of the virion resembled that of the rhabdoviruses, or in the reovirus group since the virus was ether-sensitive. It seemed necessary to create a new genus for this virus. |
| Databáze: | OpenAIRE |
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