Rapid Identification Method for Gamma-Irradiated Soybeans Using Gas Chromatography–Mass Spectrometry Coupled with a Headspace Solid-Phase Microextraction Technique
| Autor: | Beom-Seok Song, Jong-Bang Eun, Yeong-Seok Yoon, Ha-Young Park, Ki-Chang Lee, Jong-Heum Park, Jae-Kyung Kim |
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| Rok vydání: | 2020 |
| Předmět: |
Chromatography
Plant Extracts Chemistry General Chemistry Mass spectrometry Solid-phase microextraction Gas Chromatography-Mass Spectrometry Rapid identification Gamma Rays Soybeans Irradiation Gas chromatography Gas chromatography–mass spectrometry General Agricultural and Biological Sciences Solid Phase Microextraction |
| Zdroj: | Journal of Agricultural and Food Chemistry. 68:2803-2815 |
| ISSN: | 1520-5118 0021-8561 |
| Popis: | This study evaluated the applicability of a rapid analytical method using a headspace solid-phase microextraction gas chromatography/mass spectrometry (HS-SPME-GC/MS) technique to identify gamma-irradiated soybeans (0.1-5 kGy). From the partial least squares discriminant analysis used to discriminate between non-irradiated and irradiated soybean samples, 1,7-hexadecadiene was selected as the identifying marker. Response surface methodology experiments were used to determine the optimal HS-SPME extraction conditions including a carboxen/polydimethylsiloxane fiber with an extraction temperature of 98 °C and an extraction time of 55 min. 1,7-Hexdecadiene was detected in all samples irradiated at ≥ 0.1 kGy under the optimized HS-SPME-GC/MS conditions, and the unique presence of the marker in a gamma-irradiated sample was verified by comparing the results from heat, steam, microwave, sonication, and ultraviolet treatments. The comparisons of the identification properties for various conventional methods validated several advances in HS-SPME-GC/MS analysis in terms of rapid analysis, high sensitivity, and absence of solvent. |
| Databáze: | OpenAIRE |
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