Evaluation of E-cadherin/catenin complex in primary and secondary glomerulonephritis
| Autor: | Ioanna Giannopoulou, Andreas C. Lazaris, Ioannis N. Boletis, Spyros Michail, Panagiotis Davaris, Charalambos P. Stathakis, Petros M. Zeis, Lydia Nakopoulou |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Pathology
medicine.medical_specialty Delta Catenin Biology Kidney Immunoenzyme Techniques Glomerulonephritis medicine Humans Lupus Erythematosus Systemic Cell adhesion beta Catenin Kinase Cadherin Cell migration Catenins medicine.disease Cadherins Phosphoproteins Molecular biology Epithelium Cytoskeletal Proteins medicine.anatomical_structure Nephrology Catenin Trans-Activators Catenin complex Cell Adhesion Molecules alpha Catenin |
| Zdroj: | American journal of kidney diseases : the official journal of the National Kidney Foundation. 39(3) |
| ISSN: | 1523-6838 |
| Popis: | Catenins (α-, β-, γ-catenin, p120 ctn ) are cytoplasmic proteins initially identified in a complex with E-cadherin (ECD). The latter belongs to a superfamily of transmembrane glycoproteins important for cell adhesion in normal and disease states. Catenins and p120 ctn , in particular, are substrates for growth factor receptor tyrosine kinases. Cell adhesive mechanisms have an impact on cell migration and proliferation and thus are potentially involved in the pathogenesis of glomerulonephritides (GNs). Using appropriate monoclonal antibodies, we investigated the immunohistochemical expression of ECD, α-catenin, β-catenin, γ-catenin, and p120 ctn in renal biopsy specimens from 95 patients with primary GN (n = 51) and secondary lupus-associated GN (n = 44). Examined cases were divided into two groups (proliferative [n = 35] and nonproliferative [n = 60] GNs). Among examined molecules, p120 ctn , β-catenin, and γ-catenin were expressed more frequently in glomerular epithelial cells, mainly in parietal epithelium (76%, 48%, and 40%, respectively). p120 ctn and γ-catenin epithelial expression appeared to be linked closely with proliferative lupus-associated GNs ( P = 0.050 and P = 0.029, respectively). Mainly in lupus GNs, with regard to cellular crescents and epithelial cells around microadhesions to Bowman's capsule, p120 ctn (63% and 73%, respectively), β-catenin (72% and 75%), and γ-catenin (75% and 64%) showed the greatest frequencies of positive detection. Mesangial cells were positive only occasionally for the examined molecules. In proliferative lupus GNs, expression of β-catenin in mesangial cells tended to be prominent ( P = 0.066). ECD and α-catenin were not expressed in cellular crescents or microadhesions, whereas only ECD was barely detectable in glomerular epithelial cells. In conclusion, expression of β-catenin, γ-catenin, and p120 ctn is focused on glomerular epithelium, as well as on such lesions deriving from it as cellular crescents. This expression probably is linked with epithelial cells' responses to various mitogens, such as growth factors. © 2002 by the National Kidney Foundation, Inc. |
| Databáze: | OpenAIRE |
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