Primary structure of twenty three neutral and monosialylated oligosaccharides O-glycosidically linked to the human secretory immunoglobulin A hinge region determined by a combinationof permethylation analysis and 400-MHz 1H-NMR spectroscopy
| Autor: | Annick Pierce-Cretel, Geneviève Spik, Gérard Strecker, Jean-Pierre Decottignies, Jean Montreuil, Jean-Michel Wieruszeski |
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| Rok vydání: | 1989 |
| Předmět: |
chemistry.chemical_classification
Magnetic Resonance Spectroscopy Chromatography Milk Human Molecular Structure Stereochemistry Protein primary structure Oligosaccharides Secretory Immunoglobulin A Methylation Oligosaccharide Carbohydrate Mass spectrometry Biochemistry High-performance liquid chromatography Mass Spectrometry Immunoglobulin A Sugar Alcohols chemistry Humans Glycosides Trisaccharide Chromatography High Pressure Liquid |
| Zdroj: | European Journal of Biochemistry. 182:457-476 |
| ISSN: | 1432-1033 0014-2956 |
| Popis: | Sialooligosaccharide and asialooligosaccharide alditols, derived from the human milk secretory immunoglobulin A hinge region, have been purified by HPLC using, successively, an amino-bonded silica column and an octadecyl-bonded silica column. Their primary structures were completely resolved by applying a combination of sugar analysis, methylation mass spectrometry and 400-MHz 1H-NMR spectroscopy. In the present report, twenty three oligosaccharide alditols are described and all possess a type two core consisting of the branched trisaccharide: Gal(beta 1-3)[GlcNAc(beta 1-6)]GalNAc-ol. The elongation of this core arises by chain lengthening only on the GlcNAc(beta 1-6) branch for the sialylated compounds, leading to a dodecasaccharide, and on both branches for the neutral compounds leading to a nonasaccharide. Sixteen of the described oligosaccharide structures are original. Moreover, some of the fucosylated structures were found to support Le(a), Le(b) and X blood group determinants. |
| Databáze: | OpenAIRE |
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