Performance of recombinant proteins in diagnosis and differentiation of canine visceral leishmaniasis infected and vaccinated dogs
| Autor: | Elaine A. O. Assunção, Kyssia Karen de Paiva E Silva, Malcolm S. Duthie, Laura Machado Menegati, Elfadil Abass, Aimara C. Pinheiro, Ingrid Estevam Pereira, Maria De Lourdes P. Araújo, Henrique Couto Teixeira, Ulrich Steinhoff |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
diagnosis 030106 microbiology recombinant proteins kinesins rK39 canine visceral leishmaniasis Serology 03 medical and health sciences Vaccination of dogs Medicine leishmaniasis Receiver operating characteristic biology rKLO8 business.industry accuracy of diagnostic tests rLb6H Leishmaniasis medicine.disease Leishmania biology.organism_classification Virology rK28 Original Research Paper 030104 developmental biology Visceral leishmaniasis Immunization biology.protein Antibody business |
| Zdroj: | European Journal of Microbiology & Immunology |
| ISSN: | 2062-8633 |
| DOI: | 10.1556/1886.2020.00018 |
| Popis: | Control of canine visceral leishmaniasis (CVL), a major zoonotic disease in Brazil and many other tropical and subtropical countries, remains difficult as an accurate and reliable diagnosis is still missing. In endemic regions, infected dogs are the main parasitic reservoir host of human Visceral leishmaniasis (VL) infection. Vaccination of dogs against Leishmania infection constitutes an important strategy to prevent or to better control CVL, thus, a serological test that can discriminate between antibodies induced by immunization versus infection is highly desirable in order to improve and simplify diagnosis. Here, four recombinant proteins were evaluated for their ability to detect and differentiate between dogs that are infected with Leishmania or have been immunized with the anti-Leishmania vaccine Leish-Tec®. Receiver operating characteristic (ROC) curve analysis of the four Leishmania-specific IgG ELISA revealed superior performance of rK28, followed by rKLO8, rK39 and rLb6H. The rK28-based ELISA revealed not only the best accuracy against CVL, but also the lowest cross-reactivity with sera from Leish-Tec® immunized dogs. Our data show that the rK28-based ELISA is highly suitable for CVL screening as it shows high sensitivity with simultaneous low cross-reactivity. Further, the high specificity of the rKLO8 indicates its suitability for the confirmation of CVL diagnosis. |
| Databáze: | OpenAIRE |
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